- Methodology Article
- Open Access
Finding trans-regulatory genes and protein complexes modulating meiotic recombination hotspots of human, mouse and yeast
© Wu et al.; licensee BioMed Central Ltd. 2014
- Received: 10 December 2013
- Accepted: 11 July 2014
- Published: 11 September 2014
The regulatory mechanism of recombination is one of the most fundamental problems in genomics, with wide applications in genome wide association studies (GWAS), birth-defect diseases, molecular evolution, cancer research, etc. Recombination events cluster into short genomic regions called “recombination hotspots”. Recently, a zinc finger protein PRDM9 was reported to regulate recombination hotspots in human and mouse genomes. In addition, a 13-mer motif contained in the binding sites of PRDM9 is found to be enriched in human hotspots. However, this 13-mer motif only covers a fraction of hotspots, indicating that PRDM9 is not the only regulator of recombination hotspots. Therefore, the challenge of discovering other regulators of recombination hotspots becomes significant. Furthermore, recombination is a complex process. Hence, multiple proteins acting as machinery, rather than individual proteins, are more likely to carry out this process in a precise and stable manner. Therefore, the extension of the prediction of individual trans-regulators to protein complexes is also highly desired.
In this paper, we introduce a pipeline to identify genes and protein complexes associated with recombination hotspots. First, we prioritize proteins associated with hotspots based on their preference of binding to hotspots and coldspots. Second, using the above identified genes as seeds, we apply the Random Walk with Restart algorithm (RWR) to propagate their influences to other proteins in protein-protein interaction (PPI) networks. Hence, many proteins without DNA-binding information will also be assigned a score to implicate their roles in recombination hotspots. Third, we construct sub-PPI networks induced by top genes ranked by RWR for various species (e.g., yeast, human and mouse) and detect protein complexes in those sub-PPI networks.
The GO term analysis show that our prioritizing methods and the RWR algorithm are capable of identifying novel genes associated with recombination hotspots. The trans-regulators predicted by our pipeline are enriched with epigenetic functions (e.g., histone modifications), demonstrating the epigenetic regulatory mechanisms of recombination hotspots. The identified protein complexes also provide us with candidates to further investigate the molecular machineries for recombination hotspots. Moreover, the experimental data and results are available on our web site http://www.ntu.edu.sg/home/zhengjie/data/RecombinationHotspot/NetPipe/.
- Meiotic recombination hotspots
- Protein-protein interactions (PPI)
- Random walk
- Protein complexes
- Gene Ontology (GO) term analysis
- Epigenetic functions
Recombination is one of the most fundamental processes in molecular biology . It is a process that homologous chromosomes exchange their arms and such crossover events tend to occur more frequently within some short regions called “recombination hotspots”. The understanding of the mechanisms for recombination hotspots would thus shed light on various important aspects in molecular biology and medicine, such as genome instability, birth-defect diseases, disease gene mapping, molecular evolution and so on .
Recently, there has been much progress in the discovery of the mechanisms for meiotic recombination hotspots in mammalian genomes. For example, a zinc finger protein PRDM9 was reported as a trans-regulator of recombination hotspots in human and mouse genomes -. PRDM9 binds to DNA and its binding site contains a 13-mer motif previously found to be enriched in human hotspots . In , Smagulova et al. analyzed the molecular features of mouse recombination hotspots and observed that a consensus motif enriched in mouse hotspots aligns with the predicted binding site of mouse PRDM9 significantly. Using an LD-based approach named LDsplit, Zheng et al. identified HapMap SNPs (single nucleotide polymorphisms) as cis-regulators of recombination hotspots. In addition, the authors  also found an enriched 11-mer motif which closely matches the aforementioned 13-mer motif bound by PRDM9 and enriched in human recombination hotspots.
Although significant breakthroughs have been made in the understanding of the regulatory mechanisms of meiotic recombination hotspots, they are mainly focused on the well-known protein PRDM9. However, it is estimated that PRDM9 can explain only 18% of variations in human recombination phenotype . Meanwhile, the 13-mer motif contained in the binding sites of PRDM9 covers only 41% of human recombination hotspots . Therefore, PRDM9 is unlikely to be the only trans-regulator of recombination hotspots and we are highly motivated to discover other genes as trans-regulators. Recombination is such a complex process that it is unlikely to be regulated by individual proteins. Rather, multiple proteins need to act in concert as a molecular machinery to carry out the process precisely and stably, e.g., the Mre11 complex with Mre11, Rad50 and Nbs1 (also known as MRN complex) in yeast  and a FIGNL1-containing protein complex with FIGNL1 and SPIDR in human . As such, the extension of the prediction from individual proteins to protein complexes is highly desired. Furthermore, the function of PRDM9 for regulating recombination hotspots is well conserved among human, chimpanzee and mouse ,. It would be an interesting question in comparative genomics whether there are any other genes or complexes whose functional roles in regulating recombination hotspots are conserved among species.
In order to evaluate the results of our pipeline, we utilize multiple perspectives of GO term analysis. First, the GO term enrichment analysis shows that epigenetic functions are enriched in the seeds selected by various prioritizing methods. Second, we calculate the semantic similarity between identified genes and existing recombination related GO terms (i.e., GO:0006310—DNA recombination and GO:0007126—Meiosis). Genes top-ranked by RWR are demonstrated to have even higher similarities to these two particular terms than the seeds in human and yeast. This shows RWR in PPI networks is a credible complement to the existing methods since it enables the detection of novel genes without binding information. Lastly, in contrast to most existing methods which only explore the individual genes, in this paper we carry out analysis at protein-complex level and capture the underlying modularity and functional organization among those recombination related proteins.
Prioritizing genes for recombination hotspots
We first briefly introduce two different methods proposed in our previous studies for prioritizing genes for recombination hotspots, namely the Odds Ratio method (OR)  and HB network alignment method (HB) . Moreover, we present a graph-matching based method (the KM method as shown in Figure 1) in this paper to combine the results of the above two methods. As such, we can identify trans-regulators with these methods from different perspectives and later on we will conduct comprehensive comparisons among them. In addition, the terms “gene” and “protein” are used interchangeably in this paper.
Odds Ratio scores
Given a transcription factor (TF) with a binding motif, we are able to count the occurrences of this motif in hotspots and coldspots using the FIMO software . We measure the preference of the TF to bind in hotspots with the Odds Ratio O hc =(H M/H N)/(C M/C N). Here, HM is the number of hotspots with at least one motif occurrence (i.e. a hit of FIMO search), HN is number of hotspots without any hit (i.e. H N=N H -H M, where N H is the number of hotspots), CM is the number of coldspots with at least one hit, and CN is the number of coldspots without any hit (i.e. C N=N C -C M, where N C is the number of coldspots). We predict those TFs with high Odds Ratio scores, i.e., those preferring to bind to hotspots rather than coldspots, as candidates of trans-regulators of recombination hotspots. For more information, please refer to our previous study  on the Odds Ratio scores for TFs.
HB network construction and alignment for TFs
We collect the Hotspot-Binding profiles (HB profiles) for TFs. In particular, we divide the whole genome into λ bins with fixed length (e.g., 5M bases) and the HB profile of a TF g is represented as a λ−dimension vector, H B(g)=(b 1,b 2,···,b λ ), where b i is the number of hotspots in the i t h bin that g binds to. Subsequently, we can build a HB network for TFs, where a node is a TF and an edge between two TFs indicates they have similar HB profiles. The similarity between two HB profiles is measured by Pearson correlation coefficient. Two TFs are connected in the HB network when the similarity between their HB profiles is larger than a pre-defined threshold (e.g., 0.7 is used in this paper).
We construct HB networks for multiple species and apply a network comparison toolkit named NCT  to align these networks. NCT will output the conserved subgraphs among HB networks. Such cross-species alignment of HB networks can detect evolutionarily conserved network motifs associated with recombination hotspots, which are believed to be more significant than signals from single-species analysis ,. Furthermore, it is observed that proteins involved in multiple modules tend to be more biologically important . Therefore, for those TFs in HB networks, we evaluate their relevance to recombination hotspots based on their frequency in the conserved clusters collected by NCT. More specifically, for a TF g, its relevance score R(g) to recombination hotspots is measured by its frequency in the conserved clusters, i.e., the number of conserved clusters involving g, normalized by the maximum frequency over all the genes. Similar to the Odds Ratio scores, we use the above relevance scores to rank candidate genes related to recombination hotspots.
KM method to combine results from OR and HB
We build a weighted complete bipartite graph T G=(T,P,w), where T containing nodes on one side is the set of TFs and P containing nodes on the other side denotes the positions from 1 to n. There is an edge between t∈T and p∈P, denoting a possible assignment of t to rank p. The weight w(t,p)=|σ o (t)-p|+|σ h (t)-p|, denotes the footrule distance between existing rankings (σ o and σ h ) and a possible ranking that places the TF t at the position p. As such, we solve the problem in Equation 1 by finding a minimum weighted matching in TG using Kuhn-Muntres algorithm ,, and we denote this combining strategy as KM method for short.
Random walk in PPI networks
Trans-regulators can be predicted by the above prioritizing methods, i.e., OR, HB and KM. However, the power of these methods would be limited due to the small number of TFs with known binding motifs. For example, out of tens of thousands of known human and mouse genes, there are only 158 binding motifs for human and 148 for mouse in two well-known databases (i.e., JASPAR  and TRANSFAC ), respectively. Meanwhile, a large amount of protein-protein interaction (PPI) data are available and they are often modeled as graphs, where nodes are proteins and edges are interactions between proteins, for predicting novel protein interactions , protein functions , protein complexes , disease genes , etc. In this work, we exploit PPI data to evaluate the relevance of genes (proteins) to recombination hotspots, by a Random Walk with Restart algorithm (RWR) ,.
where W is the transition matrix of the PPI network and each element W ij is the transition probability from node i to node j. In this paper, the normalized adjacency matrix of the PPI network is considered as the transition matrix. The parameter γ∈(0,1) is the restart probability. At each step, the random walker may return to seed nodes with probability γ. In our experiments, it is set as 0.7 (the same as the setting in ). p ∞ (i) is the final relevance of node i to recombination hotspots. We can obtain the relevance vector at the steady state (p ∞ ) efficiently by performing iterations until the difference between p t+1 and p t is below a threshold, for example, 10-10.
Based on the RWR algorithm in PPI networks, genes that are highly interactive with the seed genes will accumulate more influence pumped from the seeds. Hence, we can consider them as novel genes associated with recombination hotspots even if they may not have known DNA binding motifs.
Predicting protein complexes for recombination hotspots
After prioritizing genes associated with recombination hot-spots, we construct sub-networks for various species, which are induced by those top-ranked genes (e.g., top 200 genes ). Therefore, we detect protein complexes highly related to recombination hotspots from these PPI sub-networks using the COACH algorithm , which predicts dense regions in PPI networks as protein complexes. Furthermore, we can detect evolutionarily conserved protein complexes involved in recombination hotspots as follows.
In previous studies ,, two protein complexes with many common proteins, which have a NA score larger than or equal to a threshold (generally set as 0.25), will be considered as the same protein complex. Similarly, a pair of super-nodes (i.e., protein complexes) in our bipartite graph CG with an edge weight larger than or equal to the threshold will be considered as a pair of conserved complexes and all the edges with weights lower than the threshold will be removed from CG. Obviously, the weight here indicates the conservation between the complexes from two species. To maximize the conservation between two species , we detect conserved protein complexes by finding maximal weighted matching in CG using Kuhn-Muntres algorithm . Finally, our conserved protein complexes are those pairs in the obtained maximum weighted matching.
GO term analysis
In this section, we briefly introduce the data used in our experiments. 3,600 yeast recombination hotspots were collected from . 39,551 human recombination hotspots were estimated from HapMap genetic map by the LDhat package . In addition, 9,874 mouse recombination hotspots were downloaded from . DNA sequences for yeast (version: sacCer3), mouse (version: MGSCv37) and human (version: GRCh37) were downloaded from NCBI.
To calculate the Odds Ratio scores and collect the HB profiles, the binding motifs of TFs were downloaded from JASPAR and TRANSFAC databases. After processing, we obtained 177 binding motifs of yeast, 158 of human and 148 of mouse, respectively. Yeast PPI data, with 17,201 interactions among 4,930 proteins, were downloaded from the DIP database . Human PPI data were downloaded from the BioGRID database , consisting of 11,120 proteins and 55,014 interactions among these proteins. Mouse PPI data were downloaded from , with 10,348 proteins and 63,882 interactions. Lastly, the GO data for various GO term analysis were downloaded from http://www.geneontology.org.
Genes ranked by various prioritizing methods
Genes ranked by Odds Ratio scores
These 18 yeast TFs include YKL112W, YBR049C, YDR026C, YHR006W, YBL103C, YFR034C, YDR463W, YBL005W, YGL013C, YDR207C, YIR023W, YDL002C, YBL054W, YER088C, YNL216W, YJR127C, YLR375W and YML081W (in the descendent order of their Odds Ratio scores). Here, YKL112W, the gene with the highest score in yeast, directly mediates a number of different chromatin-related events such as DNA replication, gene silencing, chromatin remodeling and nucleotide excision repair. In addition, YKL112W transcriptionally regulates YIL072W (HOP1), which is a meiosis-specific protein required for chromosome synapsis . YDR207C (top 10 t h gene) is a key transcriptional regulator of early meiotic genes and it couples metabolic responses to nutritional cues with initiation and progression of meiosis. In particular, it interacts with YJR094C (IME1), which is a master regulator of meiosis, to activate transcription of early meiotic genes .
The top 16 human TFs are MYC, USF1, PRDM9, PLAG1, CUX1, TFAP4, TP53, TCF3, EP300, REST, RARA, INSM1, CTCF, PAX5, SP1 and ZIC2. We observe that the known t r a n s-regulator PRDM9 (rank: 3 r d ) is captured by the Odds Ratio scores. Meanwhile, CTCF (rank: 13 t h ) is a zinc finger protein that contains 11 C 2 H 2-type zinc fingers (PRDM9 also belongs to a family of zinc finger proteins, with 14 C 2 H 2-type zinc fingers). CTCF is annotated with terms, such as GO:0031060 (regulation of histone methylation), GO:0035065 (regulation of histone acetylation) and GO:0006306 (DNA methylation), plays a critical role in the epigenetic regulation .
GO terms enriched in yeast HG genes selected by Odds Ratio method
GO term descriptions
Chromosome organization and biogenesis (sensu Eukaryota)
ATP-dependent chromatin remodeling
Ribosome biogenesis and assembly
Ribosomal large subunit biogenesis and assembly
Chromatin assembly or disassembly
Establishment and/or maintenance of chromatin architecture
Chromatin silencing at telomere
Genes prioritized by HB network alignment
We conduct GO term analysis for genes collected by the HB network alignment method (please refer to our previous study  for details on HB network construction and alignment). Due to the limited number of TF orthologs between yeast and human (or mouse), the HB network alignment method here is not applicable to the yeast TFs. We thus only show the results on human and mouse. Similarly we select top 10% TFs in each species for GO analysis, i.e., 16 out of 158 human TFs and 15 out of 148 mouse TFs are selected. In particular, these HG genes in human are SP1, PRDM9, PAX5, ESR1, CTCF, NF1, NR6A1, MYOD1, YY1, USF1, PPARG, NFKB1, MYC, RELA, REL and MYOG in the decreasing order of their relevance scores. It is observed that 6 TFs are identified by both OR and HB methods, i.e., MYC, PRDM9, SP1, CTCF, PAX5 and USF1.
GO terms enriched in human HG genes selected by HB network alignment method
GO term descriptions
Negative regulation of histone H3-K9 methylation
Regulation of histone methylation
Positive regulation of histone H3-K9 methylation
Positive regulation of histone H3-K4 methylation
Genes from the KM method
We also selected 16 HG genes prioritized by newly designed KM method. As such, the genes prioritized by OR and KM have 10 in common, while those by HB and KM have 12 in common. The GO terms enriched in the genes prioritized by the KM method are shown in Table S2 in our Additional file 1. We observed that several epigenetic terms with high gap scores are enriched in those HG genes selected by our KM method. It is interesting that the terms GO:0007283, spermatogenesis (rank: 1 s t ) and GO:0051573, negative regulation of histone H3-K9 methylation (rank: 11 t h ) in Additional file 1: Table S2 have gap scores 0.312 and 0.136, respectively. In fact, the scores for these two terms in Additional file 1: Table S2 are higher than those in Table 2 (0.243 and 0.119 respectively), indicating that the two terms are more enriched in HG genes selected by KM method than HB method.
Semantic similarity to two recombination related GO terms
HG genes (OR)
HG genes (HB)
HG genes (KM)
148 TFs with binding motifs
All mouse genes in GO
HG genes (OR)
HG genes (HB)
HG genes (KM)
158 TFs with binding motifs
All human genes in GO
HG genes (Odds Ratio)
177 TFs with binding motifs
All yeast genes in GO
Obviously, the KM method achieves the higher average GO similarities than OR and HB methods for mouse as shown in Table 3. Meanwhile, the KM method for human is moderate—OR achieves the best performance and KM is slightly better than HB. In fact, several genes have high ranks by OR while they may have low ranks by HB. As a balance, KM generally will not select them as HG genes. EP300 and TCF3 in human are indeed such cases. They have high similarity to the two recombination related terms, e.g., 0.548 for EP300 and 0.535 for TCF3, respectively. This would explain to some extent why KM does not achieve good results for human.
In addition, the HG genes in human collected by the OR method have higher similarity than those by the HB method, while the case for mouse is the opposite, i.e., HG genes collected by the HB method have higher similarity than the OR method. This demonstrates that these two prioritizing methods may have their own advantages in different species. In the same species, they may also be complements to each other. For example in human, the gene YY1 has a low Odds Ratio score while it can be identified by HB network alignment method. It is a core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair. It is annotated with the terms GO:0006310 (DNA recombination) and GO:0000724 (double-strand break repair via homologous recombination)  and is involved in recombination events by binding to DNA recombination intermediate structures .
Pathway enrichment analysis for the prioritized genes
Besides the above GO analysis, we perform pathway enrichment analysis for our prioritized genes using various tools, including DAVID  (http://david.abcc.ncifcrf.gov), EnrichNet  (http://www.enrichnet.org) and WebGestalt  (http://bioinfo.vanderbilt.edu/webgestalt/analysis.php). We feed 16 human genes prioritized by the KM method to the above three tools and obtain the following results.
First, all the three tools demonstrated that the prioritized genes are enriched in cancer pathways, as well as the apoptosis pathway and MAPK signalling pathway. Out of 16 human prioritized genes, TP53, SP1 and MYC are well-known cancer genes that play crucial rules in genome instability. This would explain why our prioritized genes are enriched in cancer pathways. In addition, 5 and 3 out of 16 prioritized genes are in MAPK signalling pathway and apoptosis pathway respectively.
Second, EnrichNet reported an interesting KEGG pathway named “Homologous recombination”, which would be associated with our prioritized genes. Although the 14 genes involved in this pathway have no overlap with the prioritized genes, we found several links between our predicted trans-regulators and the homologous recombination pathway. For example, TP53 as one of the prioritized genes interacts with BRCA2 and RAD51, which are in the pathway of homologous recombination. They work together as key components for cell cycle control and DNA repair . SP1 also interacts with BRCA2 and RAD51. Such links between the prioritized genes and the homologous recombination pathway would implicate that some of these genes are connected with recombination hotspots. The exact mechanism of such links needs further analysis, which however is beyond the scope of this paper.
Genes re-ranked by RWR
In the above subsection, the HG genes selected by various prioritizing methods (OR, HB and KM) are demonstrated to be enriched with epigenetic functions and have high similarity with two meiotic recombination-related GO terms. Here, we take them as “seeds” for the RWR algorithm in PPI networks and propagate their influence to other genes in the PPI networks, aiming to identify more genes related with meiotic recombination hotspots.
Top genes ranked by the RWR algorithm (using HG genes with high Odds Ratio scores as seeds) and their average semantic similarity to two meiotic recombination related GO terms
Top-ranked non-seeds with high GO similarities as shown in above Table 4 and Figure 3 demonstrate that they are likely to be associated with recombination hotspots. We next show some cases which play important roles in recombination hotspots. Yeast gene YJR066W (rank: 9 t h ) in Table 4 is a component of TORC1 complex and is involved in meiosis. Another yeast gene YBR160W (rank: 7 t h ) is annotated with the following GO terms: GO:0006338 (chromatin remodeling), GO:0000706 (meiotic DNA double-strand break processing), GO:0051447 (regulation of meiotic cell cycle) and GO:0010569 (regulation of double-strand break repair via homologous recombination). Human KPNA2 in Table 4 as well as Additional file 1: Tables S4 and S5 is captured by RWR algorithm. It was previously reported to be involved in recombination, with a GO annotation GO:0000018 (regulation of DNA recombination) . Human UIMC1 (rank: 1 s t ) in Additional file 1: Table S4 is a component of BRCA1-A complex . It has annotations including GO:0006302 (double-strand break repair), GO:0016568 (chromatin modification) and GO:0045739 (positive regulation of DNA repair). HDAC1 in Table 4, Additional file 1: Tables S4 and S5 is a component of the histone deacetylase complex and it is annotated with GO terms like GO:0006338 (chromatin remodeling) and GO:0006476 (protein deacetylation).
RWR in the PPI network expanded with PRDM9
As shown in the preceding section, many important genes associated with recombination hotspots can be identified by RWR in PPI networks. However, current protein interaction data for various species are still incomplete and noisy. For example, the well-known recombination regulator PRDM9 has no interaction records in BioGrid  or HPRD  databases. In order to propagate the influence of PRDM9 to other genes, its predicted interaction partners are collected from STRING  database, including SPO11, SPATA17, RNF212, H2AFX, H3F3A and H3F3B.
Top genes ranked by the RWR algorithm in an expanded PPI network “BioGrid+PRDM9” and their semantic similarity to two recombination related GO terms
Protein complexes involved in recombination hotspots
With the genes identified as seeds by various methods (OR, HB and KM), we ran RWR and constructed PPI subnetworks, e.g., with top 200 nodes. In this section, we will show the results using seeds selected by the HB method . Here, we are still not able to detect protein complexes involving the PRDM9 protein after we added its 6 interaction partners into BioGrid (i.e., SPO11, SPATA17, RNF212, H2AFX, H3F3A and H3F3B in the above section). This indicates that the expanded PPI network still does not have dense interaction structures around PRDM9. Therefore, it is desirable to find more interactions involving PRDM9 and its partners for further protein-complex detection.
where C contains k proteins in F and |V| is the total number of proteins in a given genome. A predicted protein complex with low p-values indicates that it is enriched by proteins from the same functional group and thus statistically significant. In our experiments, all the conserved complexes are predicted to be significant, i.e., with the lowest p-values lower than 0.01 . This result shows that those conserved complexes are indeed enriched by common and specific functions.
Top-5 GO terms for the conserved complex predicted by NetPipe as shown in Figure 4
In this paper, we introduced a pipeline as shown in Figure 1 to identify genes and protein complexes associated with recombination hotspots. Based on the DNA-binding information of TFs, we introduced two complementary methods, i.e., Odds Ratio scores (OR) and HB network alignment method (HB), to prioritize genes associated with recombination hotspots. We also proposed a ranking aggregation method called KM to combine the results from OR and HB. Furthermore, we exploited the PPI data to predict more proteins without binding information. Therefore, we effectively addressed the limitation that various prioritizing methods (OR, HB and KM) can only work for a small number of TFs with binding motifs. Meanwhile, we also detected protein complexes conserved between human and mouse that are associated with recombination hotspots. Evaluation results show that our pipeline is able to identify novel recombination-related genes and protein complexes. In addition, novel genes ranked in PPI networks have high semantic similarity to recombination related GO terms, showing PPI data are indeed a good source to select individual genes associated with recombination hotspots. For example, human protein KPNA2 is captured by RWR algorithm. It was previously reported to be involved in recombination, with a GO annotation GO:0000018 (regulation of DNA recombination) .
In our current results, we expanded the human PPI networks by including the interactions for PRDM9 and thus we managed to propagate the influence of PRDM9 to other proteins. However, we still cannot predict protein complexes involving PRDM9. The main reason for this issue could be that there are no dense interaction structures around PRDM9. Therefore, we may predict more interactions for PRDM9 and its partners by integrating multiple types of evidence. As such, there would be some dense structures around it, which can thus be detected by some existing density-based algorithms for protein complexes.
MW and JZ conceptualized and designed the method and drafted the manuscript together. MW was responsible for the implementation. CKK and XLL participated in discussion and conceptualization as well as revising the draft. All authors read and approved the manuscript.
This work was supported by Singapore MOE Tier 1 AcRF Grant RG32/11 (M4010977.020).
- Lichten M, de Massy B: The impressionistic landscape of meiotic recombination. Cell. 2011, 147: 267-270. 10.1016/j.cell.2011.09.038.PubMed CentralView ArticlePubMedGoogle Scholar
- Zheng J, Khil PP, Camerini-Otero RD, Przytycka TM: Detecting sequence polymorphisms associated with meiotic recombination hotspots in the human genome. Genome Biol. 2010, 11 (R103): 1-15.Google Scholar
- Baudat F, Buard J, Grey C, Fledel-Alon A, Ober C, Przeworski M, Coop G, de Massy B: PRDM9 is a major determinant of meiotic recombination hotspots in humans and mice. Science. 2010, 327 (5967): 836-840. 10.1126/science.1183439.PubMed CentralView ArticlePubMedGoogle Scholar
- Myers S, Bowden R, Tumian A, Bontrop RE, Freeman C, MacFie TS, McVean G, Donnelly P: Drive against hotspot motifs in primates implicates the PRDM9 gene in meiotic recombination. Science. 2010, 327 (5967): 876-879. 10.1126/science.1182363.View ArticlePubMedGoogle Scholar
- Parvanov ED, Petkov PM, Paigen K: Prdm9 controls activation of mammalian recombination hotspots. Science. 2010, 327 (5967): 835-10.1126/science.1181495.PubMed CentralView ArticlePubMedGoogle Scholar
- Myers S, Freeman C, Auton A, Donnelly P, McVean G: A common sequence motif associated with recombination hot spots and genome instability in humans. Nat Genet. 2008, 40 (9): 1124-1129. 10.1038/ng.213.View ArticlePubMedGoogle Scholar
- Smagulova F, Gregoretti I, Brick K, Khil P, Camerini-Otero R, Petukhova G: Genome-wide analysis reveals novel molecular features of mouse recombination hotspots. Nature. 2011, 472 (7343): 375-378. 10.1038/nature09869.PubMed CentralView ArticlePubMedGoogle Scholar
- D’Amours D, Jackson SP: The Mre11 complex: at the crossroads of DNA repair and checkpoint signalling. Nat Rev Mol Cell Biol. 2002, 3 (5): 317-327. 10.1038/nrm805.View ArticlePubMedGoogle Scholar
- Yuan J, Chen J: FIGNL1-containing protein complex is required for efficient homologous recombination repair. PNAS. 2013, 110 (26): 10640-10645. 10.1073/pnas.1220662110.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu M, Kwoh CK, Przytycka TM, Li J, Zheng J: Prediction of trans-regulators of recombination hotspots in mouse genome. Proceedings of the IEEE International Conference on Bioinformatics and Biomedicine (BIBM). 2011, IEEE, Atlanta, 57-62.Google Scholar
- Wu M, Kwoh CK, Li X, Zheng J: NetPipe: a network-based pipeline for discovery of genes and protein complexes regulating meiotic recombination hotspots. Proceedings of the ACM Conference on Bioinformatics, Computational Biology and Biomedicine (ACM-BCB). 2012, ACM, Orlando, 20-27.View ArticleGoogle Scholar
- Grant CE, Bailey TL, Noble WS: FIMO: scanning for occurrences of a given motif. Bioinformatics. 2011, 27 (7): 1017-1018. 10.1093/bioinformatics/btr064.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharan R, Suthram S, Kelley RM, Kuhn T, McCuine S, Uetz P, Sittler T, Karp RM, Ideker T: Conserved patterns of protein interaction in multiple species. PNAS. 2005, 102 (6): 1974-1979. 10.1073/pnas.0409522102.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharan R, Ideker T, Kelley BP, Shamir R, Karp RM: Identification of protein complexes by comparative analysis of yeast and bacterial protein interaction data. Journal of Computational Biology. 2005, 12 (6): 835-846. 10.1089/cmb.2005.12.835.View ArticlePubMedGoogle Scholar
- Navlakha S, Kingsford C: Exploring biological network dynamics with ensembles of graph partitions. In Proceedings of the Pacific Symposium on Biocomputing (PSB). Hawaii; 2010:166–177.Google Scholar
- Baltrunas L, Makcinskas T, Ricci F: Group recommendations with rank aggregation and collaborative filtering. Proceedings of the Fourth ACM Conference on Recommender Systems (RecSys). 2010, ACM, Barcelona, 119-126.View ArticleGoogle Scholar
- West DB: Introduction to Graph Theory, 2nd edition. 2001, Prentice Hall, Upper Saddle RiverGoogle Scholar
- Portales-Casamar E, Thongjuea S, Kwon AT, Arenillas D, Zhao X, Valen E, Yusuf D, Lenhard B, Wasserman WW, Sandelin A: JASPAR 2010: the greatly expanded open-access database of transcription factor binding profiles. Nucleic Acids Res. 2010, 38 (Database-Issue): 105-110. 10.1093/nar/gkp950.View ArticleGoogle Scholar
- Matys V, Fricke E, Geffers R, Gössling E, Haubrock M, Hehl R, Hornischer K, Karas D, Kel AE, Kel-Margoulis OV, Kloos DU, Land S, Lewicki-Potapov B, Michael H, Münch R, Reuter I, Rotert S, Saxel H, Scheer M, Thiele S, Wingender E: TRANSFAC: transcriptional regulation, from patterns to profiles. Nucleic Acids Res. 2003, 31: 374-378. 10.1093/nar/gkg108.PubMed CentralView ArticlePubMedGoogle Scholar
- Yu H, Paccanaro A, Trifonov V, Gerstein M: Predicting interactions in protein networks by completing defective cliques. Bioinformatics. 2006, 22 (7): 823-829. 10.1093/bioinformatics/btl014.View ArticlePubMedGoogle Scholar
- Chua HN, Sung W, Wong L: Exploiting indirect neighbours and topological weight to predict protein function from protein-protein interactions. Bioinformatics. 2006, 22 (13): 1623-1630. 10.1093/bioinformatics/btl145.View ArticlePubMedGoogle Scholar
- Li XL, Wu M, Kwoh CK, Ng SK: Computational approaches for detecting protein complexes from protein interaction networks: a survey. BMC Genomics. 2010, 11 (S1): S3-10.1186/1471-2164-11-S1-S3.PubMed CentralView ArticlePubMedGoogle Scholar
- Li Y, Patra JC: Genome-wide inferring gene-phenotype relationship by walking on the heterogeneous network. Bioinformatics. 2010, 26 (9): 1219-1224. 10.1093/bioinformatics/btq108.View ArticlePubMedGoogle Scholar
- Yang P, Li X, Wu M, Kwoh CK, Ng SK: Inferring gene-phenotype associations via global protein complex network propagation. PloS one. 2011, 6 (7): e21502-10.1371/journal.pone.0021502.PubMed CentralView ArticlePubMedGoogle Scholar
- Köler S, Bauer S, Horn D, Robinson PN: Walking the interactome for prioritization of candidate disease genes. Am J Hum Genet. 2008, 82 (4): 949-958. 10.1016/j.ajhg.2008.02.013.View ArticleGoogle Scholar
- Vanunu O, Magger O, Ruppin E, Shlomi T, Sharan R: Associating genes and protein complexes with disease via network propagation. PLoS Comput Biol. 2010, 6 (1): e1000641-10.1371/journal.pcbi.1000641.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu M, Li X, Kwoh CK, Ng SK: A core-attachment based method to detect protein complexes in PPI networks. BMC Bioinformatics. 2009, 10: 169-10.1186/1471-2105-10-169.PubMed CentralView ArticlePubMedGoogle Scholar
- Chua HN, Ning K, Sung WK, Leong HW, Wong L: Using indirect protein-protein interactions for protein complex prediction. J Bioinform Comput Biol. 2008, 6 (3): 435-466. 10.1142/S0219720008003497.View ArticlePubMedGoogle Scholar
- Kuchaiev O, Przulj N: Integrative network alignment reveals large regions of global network similarity in yeast and human. Bioinformatics. 2011, 27 (10): 1390-1396. 10.1093/bioinformatics/btr127.View ArticlePubMedGoogle Scholar
- Wang J, Du Z, Payattakool R, Yu P, Chen C: A new method to measure the semantic similarity of GO terms. Bioinformatics. 2007, 23 (10): 1274-1281. 10.1093/bioinformatics/btm087.View ArticlePubMedGoogle Scholar
- Pan J, Sasaki M, Kniewel R, Murakami H, Blitzblau HG, Tischfield SE, Zhu X, Neale MJ, Jasin M, Socci ND, Hochwagen A, Keeney S: A hierarchical combination of factors shapes the genome-wide topography of yeast meiotic recombination initiation. Cell. 2011, 144 (5): 719-731. 10.1016/j.cell.2011.02.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Auton A, McVean G: Recombination rate estimation in the presence of hotspots. Genome Res. 2007, 17 (8): 1219-1227. 10.1101/gr.6386707.PubMed CentralView ArticlePubMedGoogle Scholar
- Salwinski L, Miller CS, Smith AJ, Pettit FK, Bowie JU, Eisenberg D: The database of interacting proteins: 2004 update. Nucleic Acids Res. 2004, 30: 449-451. 10.1093/nar/gkh086.View ArticleGoogle Scholar
- Stark C, Breitkreutz BJ, Chatr-Aryamontri A, Boucher L, Oughtred R, Livstone MS, Nixon J, Van Auken K, Wang X, Shi X, Reguly T, Rust JM, Winter A, Dolinski K, Tyers M: The BioGRID interaction database: 2011 update. Nucleic Acids Res. 2011, 39 (Database-Issue): 698-704. 10.1093/nar/gkq1116.View ArticleGoogle Scholar
- Yellaboina S, Dudekula D, Ko M: Prediction of evolutionarily conserved interologs in Mus musculus. BMC Genomics. 2008, 9: 465-10.1186/1471-2164-9-465. [http://www.biomedcentral.com/1471-2164/9/465], [http://www.biomedcentral.com/1471-2164/9/465]PubMed CentralView ArticlePubMedGoogle Scholar
- Wu M, Kwoh CK, Przytycka TM, Li J, Zheng J: Epigenetic functions enriched in transcription factors binding to mouse recombination hotspots. Proteome Sci. 2012, 10 (Suppl 1): S11-10.1186/1477-5956-10-S1-S11.PubMed CentralView ArticlePubMedGoogle Scholar
- Gailus-Durner V, Xie J, Chintamaneni C, Vershon AK: Participation of the yeast activator Abf1 in meiosis-specific expression of the HOP1 gene. Mol Cell Biol. 1996, 16 (6): 2777-2786.PubMed CentralView ArticlePubMedGoogle Scholar
- Vershon AK, Pierce M: Transcriptional regulation of meiosis in yeast. MCurr Opin Cell Biol. 2000, 12 (3): 334-339. 10.1016/S0955-0674(00)00104-6.View ArticleGoogle Scholar
- Lee BK, Iyer VR: Genome-wide studies of CCCTC-binding factor (CTCF) and cohesin provide insight into chromatin structure and regulation. J Biol Chem. 2012, 287 (37): 30906-30913. 10.1074/jbc.R111.324962.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu S, Shi Y, Mulligan P, Gay F, Landry J, Liu H, Lu J, Qi HH, Wang W, Nickoloff JA, Wu C, Shi Y: A YY1-INO80 complex regulates genomic stability through homologous recombination-based repair. Nat Struct Mol Biol. 2007, 14 (12): 1165-1172. 10.1038/nsmb1332.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu S, Hu YC, Liu H, Shi Y: Loss of YY1 Impacts the Heterochromatic State and Meiotic Double-Strand Breaks during Mouse Spermatogenesis. Mol Cell Biol. 2009, 29 (23): 6245-6256. 10.1128/MCB.00679-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Huang DW, Sherman BT, Lempicki RA: Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2008, 4: 44-57. 10.1038/nprot.2008.211.View ArticleGoogle Scholar
- Glaab E, Baudot A, Krasnogor N, Schneider R, Valencia A: EnrichNet: network-based gene set enrichment analysis. Bioinformatics. 2012, 28 (18): i451–i457-10.1093/bioinformatics/bts389.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhang B, Kirov S, Snoddy J: WebGestalt: an integrated system for exploring gene sets in various biological contexts. Nucleic Acids Res. 2005, 33 (suppl 2): W741–W748-PubMed CentralPubMedGoogle Scholar
- Marmorstein LY, Ouchi T, Aaronson SA: The BRCA2 gene product functionally interacts with p53 and RAD51. Proc Natl Acad Sci. 1998, 95 (23): 13869-13874. 10.1073/pnas.95.23.13869.PubMed CentralView ArticlePubMedGoogle Scholar
- Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA: Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein. PNAS. 1994, 91 (13): 6156-6160. 10.1073/pnas.91.13.6156.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang B, Matsuoka S, Ballif BA, Zhang D, Smogorzewska A, Gygi SP, Elledge SJ: Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science. 2007, 316 (5828): 1194-1198. 10.1126/science.1139476.PubMed CentralView ArticlePubMedGoogle Scholar
- Keshava Prasad TS, Goel R, Kandasamy K, Keerthikumar S, Kumar S, Mathivanan S, Telikicherla D, Raju R, Shafreen B, Venugopal A, Balakrishnan L, Marimuthu A, Banerjee S, Somanathan DS, Sebastian A, Rani S, Ray S, Harrys Kishore CJ, Kanth S, Ahmed M, Kashyap MK, Mohmood R, Ramachandra YL, Krishna V, Rahiman BA, Mohan S, Ranganathan P, Ramabadran S, Chaerkady R, Pandey A: Human protein reference database - 2009 update. Nucleic Acids Res. 2009, 37 (Database-Issue): 767-772. 10.1093/nar/gkn892.View ArticleGoogle Scholar
- Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A, Lin J, Minguez P, Bork P, von Mering C, Jensen LJ: STRING v9.1: protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res. 2013, 41 (Database-Issue): 808-815. 10.1093/nar/gks1094.View ArticleGoogle Scholar
- Sandor C, Li W, Coppieters W, Druet T, Charlier C, Georges M: Genetic Variants inREC8,RNF212, andPRDM9, Influence Male Recombination in Cattle. PLoS Genet. 2012, 8 (7): e1002854-10.1371/journal.pgen.1002854. [http://dx.doi.org/10.1371%2Fjournal.pgen.1002854], [http://dx.doi.org/10.1371%2Fjournal.pgen.1002854]PubMed CentralView ArticlePubMedGoogle Scholar
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