- Research article
- Open Access
Short linear motifs in intrinsically disordered regions modulate HOG signaling capacity
BMC Systems Biologyvolume 12, Article number: 75 (2018)
The effort to characterize intrinsically disordered regions of signaling proteins is rapidly expanding. An important class of disordered interaction modules are ubiquitous and functionally diverse elements known as short linear motifs (SLiMs).
To further examine the role of SLiMs in signal transduction, we used a previously devised bioinformatics method to predict evolutionarily conserved SLiMs within a well-characterized pathway in S. cerevisiae. Using a single cell, reporter-based flow cytometry assay in conjunction with a fluorescent reporter driven by a pathway-specific promoter, we quantitatively assessed pathway output via systematic deletions of individual motifs. We found that, when deleted, 34% (10/29) of predicted SLiMs displayed a significant decrease in pathway output, providing evidence that these motifs play a role in signal transduction. Assuming that mutations in SLiMs have quantitative effects on mechanisms of signaling, we show that perturbations of parameters in a previously published stochastic model of HOG signaling could reproduce the quantitative effects of 4 out of 7 mutations in previously unknown SLiMs.
Our study suggests that, even in well-characterized pathways, large numbers of functional elements remain undiscovered, and that challenges remain for application of systems biology models to interpret the effects of mutations in signaling pathways.
There is an increasing proportion of known signaling interactions mediated by regions of proteins lacking a defined tertiary structure under native conditions, known as intrinsically disordered regions (IDRs). Comparative proteomics studies have used evolutionary conservation to identify short functional elements in these intrinsically disordered regions  and suggest that many remain undiscovered . The short conserved elements identified are often short linear motifs (SLiMs), which are ubiquitous and functionally diverse elements (3–10 amino acids), and are often (~ 80%) located within the IDRs . Unlike well-defined large, globular domains, the functional significance of these motifs is inherently difficult to determine due to their small size and relatively low binding affinities  .
One of the best-understood functions of SLiMs is to mediate signaling interactions, both through post-translational modifications and peptide-domain interactions [5,6,7,8,9]. Recent evidence also suggests “weak” SLiMs in disordered regions can facilitate/recruit post-translational modifications . Components of well-characterized model signaling pathways, such as the high osmolarity glycerol (HOG) MAPK pathway in budding yeast [11,12,13,14,15], contain numerous short conserved elements (predicted short linear motifs, pSLiMs) within their IDRs. This suggests that, even in well-studied pathways, there are still many uncharacterized elements to be discovered. Here we set out to test this prediction.
Because each SLiM is thought to mediate a single, usually transient interaction, we expect mutations in them to have quantitative effects on protein function. We therefore decided to employ a quantitative, single cell reporter assay [16, 17] to detect subtle differences in activation of the pathway. Since the HOG pathway has been the subject of detailed modeling [18,19,20,21,22], we attempted to interpret the effects of mutations within mechanistic, quantitative models.
Overall, we identified 7 previously uncharacterized short protein sequences (between 3 and 17 amino acids in length) that significantly impact pathway output when deleted. Among these and 3 previously known motifs, we observed a wide quantitative range of effects on pathway output and tested whether these effects could be explained by perturbations of parameters in a previously proposed quantitative model of the HOG pathway . We found that the model can quantitatively reproduce the effects of 3 of 10 of the mutations, but in general it cannot identify the part of the pathway affected by the mutation. For mutations with the largest effects, the model agrees with the data only qualitatively. Although it is increasingly appreciated that disordered regions mediate important signaling interactions, our study highlights that systematic approaches are needed to identify the large number and quantitative effects of these interactions. We suggest that interpretation of the quantitative effects of mutations using mechanistic models is an important area for further research in systems biology.
Well-characterized signaling pathways contain short conserved elements in disordered regions
We searched for short motifs in disordered regions in model signaling pathways in yeast using previously described bioinformatics methods . We considered all predicted short conserved elements to be pSLiMs for the purpose of this study. The HOG pathway is a well-characterized and highly conserved MAPK pathway [11,12,13,14,15]. In budding yeast this pathway responds to increased external osmolarity, altering metabolism, cell cycle progression and gene expression to re-establish cell homeostasis. The pathway consists of three MAPKK’s comprising two partially redundant incoming branches (Sln1-dependant and Sho1-dependant branches)  that function in parallel, eventually converging at the MAPKK (Pbs2) [12,13,14, 18]. Activated Pbs2 in turn phospho-activates Hog1p which then translocates to the nucleus, initiating transcription of up to 600 genes [12, 13]. Here we focus on the Sho1-dependant branch of the pathway which can be studied in isolation by deleting the two redundant MAPKK’s of the Sln1-dependant branch [24, 25].
Analysis of the Sho1-dependant branch of the HOG pathway yielded 29 predicted short linear motifs (pSLiMs) within 14 disordered regions (encompassing 147 residues, or 1.9% of the amino acids in the proteins in this branch of the pathway, Fig. 1). Among these short conserved elements, at least 4 correspond to known short linear motifs in this pathway.
Because the HOG pathway (particularly the Sho1 branch) has been the subject of extensive molecular characterization [26, 27] and detailed quantitative analysis and modeling [12, 13, 18], we were surprised to identify 25 short conserved elements in disordered regions for which we could not identify functions in the literature. To confirm that the HOG pathway was not unusual in this regard, we examined another well-characterized yeast pathway, as well as a human pathway, and observed similar conserved putative motifs in both. The cell wall integrity pathway in yeast  contains 36 total pSLiMs in 11 proteins (Additional file 1: Table S1A), and the human canonical Wnt regulatory pathway  contains 23 pSLiMs in 14 proteins (pSLiMs predicted in human , Additional file 1: Table S1B). This indicates that many signaling pathways may contain large numbers of uncharacterized short linear motifs (see discussion).
Mutations in known short motifs affect HOG signaling
We sought to test for functional importance of the short, conserved elements (pSLiMs) in the HOG signaling pathway. We first confirmed that previously characterized pSLiMs in the HOG pathway affected signaling function in a reporter-based flow-cytometry assay that can quantitatively measure pathway activity using a fluorescent reporter downstream of Hog1- specific transcriptional output (see Methods). We made deletions and point mutations in the conserved Sho1 SH3 domain-binding site in Pbs2 (the MAP2K, Fig. 1) [26, 27], as well as deletions in a known motif in Hot1 (a downstream transcription factor) , a known Ste50 binding site in Opy2 (membrane anchor for Ste50p)  and the Bem1 binding site in Ste20 .
We found that when deleted, three of these four known motifs showed reduction in reporter activity (Fig. 2). This confirms that our assay has power to detect the effects of mutations of known signaling interactions mediated by short protein sequences in disordered regions. Deletion of the proline-rich SH3-binding site in Pbs2  virtually abolished the signaling response (Fig. 2a). To confirm that the strong effect observed in the Pbs2 deletion was due to the specific signaling interaction, and not a global effect on protein function due to deletion of the binding site, we also measured the signaling response in the presence of two point mutations (residues P96 and P99) that were previously shown to compromise binding . As expected, signaling in this two-point proline mutant showed a signaling response that was similar to that of the binding site deletion (Fig. 2a).
Because our flow cytometry assay measures reporter activity in single cells, we compared the distribution of reporter activity measurements for mutant strains to wild-type using the Kullback-Leibler Divergence (KL divergence, see methods). If two distributions are identical, the measure gives a value of zero; any real data will have positive KL divergence because of experimental (and possibly biological) noise. To understand how much KL divergence we could expect due to these (non-genetic) sources, we compared the distribution of wild-type reporter measurements to themselves (see methods) and found on average KL = 0.016 (+/− 0.014 standard deviation, n = 38). To assess whether a mutant affected HOG signaling, we compared the divergence (in replicate experiments) of the mutant distribution to that of the wild-type. If the divergence of the mutant to the wild-type was significantly greater than the wild-type compared with itself, we consider that mutant to impact signaling (see Methods for more details).
Using this approach, we found the deletion of a known Ste50 binding site in Opy2 showed a small, but statistically significant quantitative effect (median KL divergence, 0.070 vs. 0.012, Wilcoxon Rank Sum (WRS), n = 6 vs. 38, P < 0.001, corrected, Additional file 2: Table S2, Fig. 2) on reporter expression. This is notable, as this binding site is one of three cooperative motifs that are required for the full Ste50-Opy2 interaction , indicating that our quantitative assay may be sensitive enough to identify individual components of other cooperative binding interactions.
Unlike the binding site in Opy2, deletion of the known Bem1 binding site in Ste20  showed no significant effect on reporter expression (Additional file 2: Table S2). This motif is known to be involved in yeast mating signaling , and its functional effect would likely be detected using a different assay. This shows that our assay cannot be expected to detect all functional motifs in HOG pathway components (see discussion).
Perturbation of Hot1 activity is known to lead to stochastic responses in the HOG pathway . Interestingly, and consistent with previous studies implicating Hot1 with stochastic responses, we found that deletion of the known Hog1 binding region in Hot1 (residues 373–385; Alepuz et al., 2003) led to a bimodal response (1.526 vs. 0.012, WRS, n = 11 vs. 38, P < 0.00001, corrected, Additional file 2: Table S2, Fig. 2) where some of the cells showed no difference from the uninduced state, while others showed a weak response (Fig. 2). These results confirm that our assay can detect both quantitative, population level as well as stochastic, single cell level effects on signaling. Furthermore, these results suggest that mutation of a short conserved binding site can enhance stochastic responses (see discussion).
7/13 previously unknown short conserved elements in disordered regions quantitatively decrease HOG signaling
Using the same assay as above, we tested a cohort of previously uncharacterized pSLiMs in the disordered regions of the HOG pathway (Fig. 1). Of the predicted novel motifs within the pathway, we selected 15 candidates for functional analysis based our examination of the alignments with preference given to pSLiMs in the kinases and other signaling proteins. We created 13 yeast strains in which we deleted an individual pSLiM of at least 4 residues and a single strain (Pbs2 Δ292–98) in which two small adjacent pSLiMs (Pbs2 292–3 and 295–6) were deleted for a total of 14 strains. We then quantified pathway output of each strain and compared it the wild-type (Additional file 2: Table S2). For one of the mutants (Sch9 Δ279–95) we were not able to obtain strains with consistent pathway output, apparently due to secondary mutations. We therefore excluded it from further analysis. Of the remaining 13 strains, 7 (53.8%) showed changes in the reporter distribution (Fig. 3; Additional file 2: Table S2; WRS; P < 0.05).
These varied effects include nearly complete abolishment of the pathway activity for deletion of a previously uncharacterized proline-rich sequence in Pbs2, while most mutants showed subtle quantitative effects. The large effect of the mutation in the Pbs2 pSLiM is consistent with Pbs2’s role in localizing the signaling complex [13, 27, 31], but we were surprised that this seemingly critical motif was not identified before. That most of the pSLiMs show quantitative effects is consistent with our expectation that they are involved in transient, possibly combinatorial regulatory interactions, much like the known motifs in Opy2. Taken together, this data indicates that the novel motifs show a varied functional significance similar to the previously known motifs (Fig. 2).
Comparison of pSLiM mutants to a stochastic model of HOG signaling
In principle, quantitative models of signaling can be used to connect genotype to phenotype, and could therefore be used to predict the impact of mutations in disordered regions. Considering that signaling proteins are often mutated in human disease, this represents a potentially important area of application for quantitative models.
The yeast MAPK signaling pathways have served as a foundation for the understanding of signaling in more complex organisms , and the HOG pathway in particular has been the subject of several mechanistic modeling studies [18, 20,21,22, 34,35,36]. Previous modeling studies of the HOG pathway have demonstrated that the distribution of quantitative single cell reporter measurements can be explained using stochastic simulations of models of signaling and gene expression [20, 21, 36]. However, few studies have explored the extent to which these models generalize to new datasets, nor have they considered whether models can capture quantitative effects of mutations.
We therefore first tested whether a previously published model of HOG signaling  could explain the reporter expression we observed for the wildtype strain. We simulated individual cell trajectories and normalized the output of the model to convert the scale (in molecules) to fluorescence and computed the KL divergence between the distribution predicted by the model and the real data. We found that the model at stress level 18 (with other parameters unchanged) could quantitatively reproduce the wild-type data observed in reporter assay (Fig. 4a), such that the KL divergence between the model and the observed data was 0.061 (+/− 0.03431 standard deviation, n = 38), which is larger, but on the same order as the divergence between replicates (0.016 +/− 0.014). This indicates that the previously developed model (with appropriate normalization) can explain the distribution of reporter expression in our experimental setup.
To predict the effect of mutations in the pathway, we assumed that each mutation would affect only one of the 5 parameters associated with signaling components in the model (i.e., we did not consider parameters that control global rates of transcription or translation because our mutations were made within the signaling components). Because the simplified model does not explicitly include signaling components (or their protein interactions) we do not know in advance which parameter each mutation would affect, nor do we know the magnitude of the effect the mutation in a SLiM would have on the parameter. However, through simulations of where the different parameters were reduced individually (to model the effects of mutations), we noticed that the effects on the parameters fell into two groups (Additional file 3: Figure S1), based on whether the parameters affected the pathway activation steps before Hog1 (which we refer to as activity parameters) or the slow transcriptional steps below Hog1 (which we refer to as remodeling parameters). This apparent redundancy in the effects of perturbations to the parameters greatly simplified our analysis of mutant pathways: we need only compare our data to two sets of perturbed simulations. Nevertheless, for each type of parameter, we don’t know what quantitative effect the pSLiM mutation will have.
To evaluate the fit of the perturbed model to the reporter expression data from the mutant signaling pathways, we identified the parameter value that minimized the KL divergence between the mutant data and the prediction of the perturbed model. For example, for the known binding site in Opy2, we found that a model where the activity parameter was reduced by 50% fit the mutant data with KL divergences comparable to the fit of the unperturbed model to the wild-type data (Fig. 4b). Indeed, the KL divergences between this perturbed model and the Opy2 mutant data were significantly smaller than those between the Opy2 mutant data and the wild-type model (0.064 vs. 0.070, WRS, n = 6 vs. 6, P < 0.02, corrected) which is expected in part because the mutant model has added flexibility. Interestingly, we could not achieve a significantly better fit for the models when the remodeling parameter was perturbed (P = 0.4, WRS, corrected) suggesting that the mutation in the Opy2 binding site affects the signal activation upstream of Hog1, which is consistent with the known position of Opy2 in the pathway (Fig. 1). To test this hypothesis directly, we compared the divergences of the best perturbed activity parameter model to the best perturbed remodeling parameter model, but we found that the divergences between the data and the two perturbed models were not significantly different. Nevertheless, this analysis suggests that perturbations of single parameters in the HOG model can explain the quantitative effects of the pSLiM deletion mutants.
We applied this analysis systematically to test which other mutant data could be explained by the model. For four of the seven previously unknown pSLiM deletion mutants that had significant effects on pathway output (shown in Fig. 3), we found the fit of the perturbed model to the mutant data was comparable to the fit of the wild type model to the wild type data (Fig. 5). Considering that this model was not designed to fit quantitative data, nor to model effects of mutations , this generalization capacity is impressive. For the remaining three mutant strains the divergences of the perturbed model were significantly larger. We noted that these three strains represented the mutants with the largest effects (see discussion). We attempted to use the fits of the model to predict which parameter the pSLiM mutation affected, and although in some cases the fit of a perturbed model with one parameter or the other was slightly better, these differences were not significantly different (e.g. Fig. 6a, See discussion).
We also noted for mutations with large effects, the model tended to over predict the population variation. For example, for Sch9 and Hot1 mutants, the best fitting models produce bimodal distributions where the two peaks are more separated than observed (Fig. 6b). Further, for these mutations, the parameter that gives the best fitting mutant model is the activity parameter, which is inconsistent with the known roles of these proteins in the transcriptional part of the pathway.
Thus, by perturbing the parameters in the model to fit the pSLiM mutant data we can identify changes in parameters that improve the fit of the model to the data. However, it does not imply that the model is providing mechanistic insight into to the impact of the mutation on signaling function (see Discussion).
The use of stochastic models in systems biology is still a largely qualitative endeavor, consistent with recent research efforts indicating that fitting stochastic models to data remains a challenge in systems biology [20, 37]. To fit a previously published model  to our data, we assumed that mutations quantitatively effect a single parameter, performed simulations assuming all possible values of that parameter, and chose the value that minimizes the KL divergence of the simulation from data. Simulations revealed that many of the parameters had similar effects on the output pathway, suggesting that these parameters cannot be inferred from our experimental data . Encouragingly, we found that perturbed versions of the previously reported model could explain the distribution of signaling output. However, in general, we could not infer which parameter was affected by a given mutation, as both parameters were usually able to maximize the fit of the model to the data. This suggests that interpretation of mechanisms of mutations using systems biology models is an area requiring further research.
In addition, we noted that the model was better able to match the distributions for mutations that had small effects, and even for these mutants, the perturbation to the parameter needed to achieve these fits was usually ~ 50%. Thus, despite the small effects observed in our signaling assay, the effects on the underlying biomolecular reactions are inferred to be substantial. We speculate that this might explain the evolutionary conservation of motifs that appear to be dispensable. This suggests that the pSLiMs are required for the efficiency of signaling, but they are not required for the reactions to occur. For mutants that showed large effects in our assay, the perturbed models tended to over predict the variation, which we believe is due to the simplicity of the model.
An example of a large effect mutation is the deletion of a known motif in Hot1 . Consistent with a recent report that regulation of Hot1 by Casein Kinase (CK2) affects the stochasticity of the signaling response , we found that mutation of the known short motif in Hot1 also leads to a bimodal response, supporting the model that stochastic responses in this signaling pathway originate at the level of transcriptional regulation. To our knowledge, our results for the effects of deletion of a short motif in Hot1 is the first demonstration that short motifs in disordered regions can modulate the stochastic response of a signaling pathway [20, 36].
Because we identified new motifs in the disordered regions of a well-characterized signaling pathway in yeast, our results suggest that most signaling pathways probably contain large numbers of unappreciated functional elements in disordered regions. We only investigated one type of osmotic stress at a single concentration and tested only single motif deletions, which overlook the possibility of cooperative interactions between weak motifs . Furthermore, HOG pathway components take part in other signaling networks. For example, the known SH3 binding site in Ste20 [32, 39] was not identified, likely because this interaction is involved in mating signaling and not HOG signaling. Therefore, it’s likely that additional exploration will uncover functions for the pSLiMs that showed no effects in our assay.
If we can extrapolate from our results on the HOG pathway where we found 10 out of 29 (34%) pSLiMs had significant effects on pathway output. We predict a large fraction of pSLiMs found in other signaling pathways would also be functionally significant. As an example, we applied the same phyloHMM software  to predict candidate motifs in another MAPK pathway in yeast and found 36 pSLiMs in the cell wall integrity pathway  (Fig. 7, left panel and Additional file 1: Table S1). We suggest that at large number of these are functional. An analysis of human proteins  identified 24 pSLiMs in the human canonical Wnt signaling pathway  (Fig. 7 right panel and Additional file 1: Table S1), of which many would be important for signaling. Taken together, this suggests that there is still a large number of short motifs to be discovered in well-studied signaling pathways  and that comparative proteomics methods will be powerful tools to focus discovery efforts [2, 29, 43,44,45].
Here we have focused on short motifs because some of these can be identified using comparative proteomics approaches [2, 29, 43,44,45]. However, we note that our comparative proteomics prediction of short conserved elements in the HOG pathway is likely to have missed important functional elements  -- the number of unidentified motifs in disordered signaling molecules is likely even larger. Furthermore, in addition to short conserved SLiMs, there are many other types of functions that disordered regions may mediate in signaling pathways [7, 8, 46]. Reliably identifying the currently uncharacterized functions of disordered regions will likely be critical to fully characterizing the mechanisms of signaling in human pathways. Indeed, approximately 22% of human disease mutations are now known to occur in IDRs , thereby implicating both IDRs and the undiscovered SLiMs (and other functional elements they contain) in any mechanistic understanding of human disease.
Yeast strains and mutagenesis
HOG pathway flow cytometry assays were all performed in BY4741 (S288C-derivative strain: MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) lacking the Sho1-independent branch of the osmoresponse pathway (ssk2Δ, ssk22Δ) [22, 48]. The GFP-based reporter for the flow cytometry assay contained 800 bp of the native Stl1 promoter controlling expression of the yeast-enhanced yEGFP , integrated into the HO locus (SSK2 Δ::HisMX3 SSK22 Δ::0; HO Δ::STL1pr-GFP, LEU2). All subsequent deletions/mutations of the genomic sequence were performed using the Delitto Perfetto method for seamless mutagenesis of the yeast genome , and were verified by sequence analysis. The Positive control strain for the flow cytometry assay was created by deleting the conserved Sho1 Sh3 domain-binding site (Δ91–99) in Pbs2  . A secondary, more conservative control strain was created using double point mutations P96A and P99A  essential to the proline-rich Sh3 domain-binding site (PXXP). In order to address any possible consequence of the SLiM deletions on protein expression and localization, we selected three deletions that showed significant effect on pathway output (PBS2 Δ180–188; PBS2 Δ269–274; STE50 Δ341–346) and created C-terminal fusions with a fluorescent tag (mCherry) of each deletion (and the corresponding wild-type counterpart) for subsequent confocal analysis before and after induction of the HOG pathway. The mCherry tag was amplified in conjunction with the Ura3 auxotrophic marker and integrated as a fusion into each designated strain. Confocal analysis of fixed (see below) uninduced and induced cells revealed no difference in either expression or localization as compared to wild-type (Additional file 4: Figure S2).
Pathway induction and flow cytometry assay
HOG pathway induction experiments were performed as follows: Replicate overnight cultures inoculated from single colonies were diluted 10-fold into the appropriate selective media and grown to mid-log phase (4 h @ 30 °C). Un-induced samples were taken at this point, and cells were pelleted and re-suspended in an equivalent volume of fixative (1X PBS, pH 7.0; 0.5% paraformaldehyde). Cultures were then induced by addition of NaCl to a final concentration of 0.4 M and incubated @ 30 °C for an additional 60 min. Induced samples were then taken and treated as above. All fixed samples were stored @ 4 °C pending flow cytometry analysis (within 48 h).
Reporter activation in fixed cells from all induction experiments was subsequently measured by flow cytometry. Flow cytometry analysis was performed on a BD FACSAria IIU High Speed Cell Sorter, incorporating 3 air-cooled lasers at 488, 633, and 407 nm wavelengths, and equipped with BD FACSDiva™ software (v. 6.1.3). The instrument was calibrated prior to each experiment using a blend of size-calibrated fluorescent beads. The GFP fluorescence was excited at 488 nm and collected through 530/30 nm bandpass filter. All samples were further diluted by a factor of 10, and a total of 50,000 events (cells) were counted for every sample within gated SSC and FSC populations that excluded doublets and small debris. Typical acquisition rates were 700–900 events/second. Initial flow cytometry data was analyzed using FLowJo software (v 10, OSX).
Histogram data analysis
Data analysis of flow cytometry data was performed using in-house Matlab scripts. We used KL divergence to compare distributions. Because KL divergence is based on continuous probability distributions, to implement it on discrete data set, we used an approximation to estimate the KL divergence based on the empirical distribution .
Comparison of mutant distributions to wild type distribution
We pooled WT data from the same experiment day (3 to 4 replicates) which we refer to as the WT pool. We then used this WT pool as the Q distribution to calculate KL divergence. Used in this way, the KL divergence can be thought of as the error one would make if the wild type distribution were used in place of a given mutant distribution. The KL divergences of each WT replicate from the WT pool of the same experiment day is calculated and grouped to estimate the variance in WT reporters. We then calculated the KL divergence of a mutant replicate from the WT pool of the same experiment day. The divergences of the same mutant from different experiment days are then grouped together. We tested if the mutants’ divergences from WT pools are different from WT’s divergences from WT pools with the Wilcoxon Rank Sum test (WRS) and Bonferroni correction for multiple tests (17 tests, Additional file 2: Table S2).
A stochastic model of HOG transcriptional activation
We implemented the stochastic model  using SimBiology in MATLAB. The model can be summarized into two steps: Hog1 activation and gene activation. Hog1 is activated through a basal phosphorylation rate, and the phosphorylation rate increases when there is stress. Phosphorylated Hog1 will either be dephosphorylated by a phosphatase, or promote the steps of gene activation. Gene activation requires four components to form a complex, which are a transcription factor, an activated Hog1, a polymerase, and a chromatin remodeler. Once the gene is activated, it will be transcribed until Hog1 is deactivated by a phosphatase. Transcribed mRNA will be degraded, but the protein translated from the mRNA accumulates until the end of the simulation, t = 3600 s. This accounts for the stability of GFP on the timescales considered here. The reactions, stochastic rate parameters, and initial amounts of species are listed in Additional file 2: Table S2 and Table S3 from the original paper .
The value of the stress parameter was set to 18 at t = 0 s, which minimizes the KL divergence from our wild-type pools under 0.4 M NaCl stress after 60 min. We modeled the effects of mutation by decreasing the value of 2 parameters, one that determines the activation of Hog1 by stress, the other determines the frequency that remodeler binds to DNA. We varied each parameter independently, in increments of 5% of the original value. In total, 40 perturbations (20 per parameter) were performed with 50,000 runs to represent 50,000 cells of each.
The distribution obtained for each perturbation was analyzed using the same method that we used to compare the mutant distributions to the wildtype distribution (described above) with the following modifications: first, a small lognormally distributed constant (mu, sigma = 2.5, 0.6) was added to the results of each run to represent basal gene expression (rather than normally distributed constant as assumed previously ). Second, the simulated data were linearly transformed such that two requirements are satisfied: 1) the maximum intensity was aligned to a replicate of WT data (which subsequently excluded for comparisons of KL divergence). 2) The mean of unstressed simulated distribution was aligned to the mean of unstressed control for the particular experimental replicate being compared. The maximum and minimum intensity of the experimental replicate were introduced into the simulated distribution as a form of linear extension .
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Thanks to Bianca Garcia and Dr. Alan Davidson for discussions and technical assistance. We thank Drs Audrey Gasch, Eric Weiss, Julie Forman-Kay, Yihan Lin and Muluye Liku for discussions and comments on the manuscript.
ANNB and TZ were funded by postgraduate scholarships from the Natural Sciences and Engineering Research Council of Canada (NSERC). This research was supported by a Canadian Institutes of Health research (grant MOP-119579) and by infrastructure grants from the Canadian Foundation for Innovation to AMM.
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Table S1. Predicted short linear motifs (pSLiMs) in the hypotonic stress and canonical Wnt signaling pathways. A. Signaling components of the hypotonic stress pathway in S.cerevisiae identified from KEGG, with 36 SLiMs predicted in 11 hypotonic stress signaling proteins using PhyloHMM. B. Signaling components of the human canonical Wnt signaling pathway identified from KEGG, with 24 SLiMs predicted in 16 Wnt signaling proteins using SLiMPrints. Periods indicate positions where any amino acid can match. (PDF 32 kb)
Table S2. P-values from Wilcoxon Rank Sum Tests comparing the distribution of KL divergence from mutants to wild-type pools. (PDF 37 kb)
: Figure S1. Effects of perturbations in model parameters fall into two groups. The activity parameter that affects the pathway activation steps upstream of the MAPK Hog1 (referred to as c_active in , top panel) affects both the proportion of cells that activate the pathway (right mode of distribution) as well as the maximum activity of the pathway (location of the right mode), such that as the parameter is reduced, fewer cells activate the pathway and those that do, do so to a lesser extent. The bottom panel shows that the four parameters that affect the slow transcriptional steps below Hog1 (referred to as c_tfon, c_hogon, c_remon, c_polon in ) affect the proportion of cells in the right mode, but not the location of the mode, so the distributions appear more bimodal. Each plot contains predictions of the model where the original parameter value has been permuted in the range from 0 to 50% of the original parameter value in increments of 5%. (TIF 736 kb)
: Figure S2. Controls for mutant protein expression. Confocal images of dual fluorescent reporter strains with a pSTL1::GFP HOG pathway reporter combined with C-terminal mCherry fusions of Pbs2 Δ180–188, Pbs2 Δ269–274, Ste50 Δ341–346, wild-type Pbs2 and wild-type Ste50 before and after a 60 min induction with 0.4 M NaCl. The top row shows the pSTL1::GFP wild-type strain (no mCherry) for comparison. The left two columns show the mCherry channel, and we find no difference of mCherry fusion protein expression levels or localization in SLiM deletion strains as compared to their wild-type counterparts. The right two columns show the GFP channel and confirm that the HOG pathway has been induced as expected (based on our flow cytometry results) in the mCherry tagged strains. (TIF 1835 kb)
About this article
- Cell signaling
- Saccharomyces cerevisiae
- Mitogen-activated kinases
- Intrinsically disordered regions
- Short linear motifs
- High osmotic glycerol pathway