 Methodology article
 Open Access
 Published:
A specialized ODE integrator for the efficient computation of parameter sensitivities
BMC Systems Biology volume 6, Article number: 46 (2012)
Abstract
Background
Dynamic mathematical models in the form of systems of ordinary differential equations (ODEs) play an important role in systems biology. For any sufficiently complex model, the speed and accuracy of solving the ODEs by numerical integration is critical. This applies especially to systems identification problems where the parameter sensitivities must be integrated alongside the system variables. Although several very good general purpose ODE solvers exist, few of them compute the parameter sensitivities automatically.
Results
We present a novel integration algorithm that is based on second derivatives and contains other unique features such as improved error estimates. These features allow the integrator to take larger time steps than other methods. In practical applications, i.e. systems biology models of different sizes and behaviors, the method competes well with established integrators in solving the system equations, and it outperforms them significantly when local parameter sensitivities are evaluated. For easeofuse, the solver is embedded in a framework that automatically generates the integrator input from an SBML description of the system of interest.
Conclusions
For future applications, comparatively ‘cheap’ parameter sensitivities will enable advances in solving large, otherwise computationally expensive parameter estimation and optimization problems. More generally, we argue that substantially better computational performance can be achieved by exploiting characteristics specific to the problem domain; elements of our methods such as the error estimation could find broader use in other, more general numerical algorithms.
Background
In systems biology, mathematical models often take the form of system of ordinary differential equations (ODEs). These are approximations of the underlying mechanisms such as enzymecatalyzed biochemical reactions that are applicable when molecule numbers are sufficiently high, and when the spatial distributions of components in a cell can be neglected. More specifically, ODE models consider the rate of change in a set of states (e.g. species concentrations) as a function of the system’s current state, its inputs, and its inherent kinetic parameters that capture, for instance, affinities of molecular interactions [1].
In contrast to systems modeling in domains such as physics, however, model parameters and initial conditions for systems biology models are often not known, or they can only be roughly approximated. As few kinetic parameters can be measured directly, parametric uncertainty often prevails [2]. How the system variables depend on these system parameters can therefore be of interest, e.g. to help find parameters such that the simulated system matches some observed or desired behavior. Dependencies between system variables and parameters are captured by the local parameter sensitivities that describe to what extent the state of the system changes when parameter values are perturbed from a reference value. Formally, local parameter sensitivities comprise the set of derivatives of all system variables with respect to the system parameters. As with the state dynamics, the parameter sensitivities’ time evolution follows a system of ODEs [3].
From a computational point of view it is important to note that in all but the simplest cases, starting from a set of initial conditions, there is no direct way to compute the solutions of a system of ODEs (states or parameter sensitivities in our case) for an arbitrary time. The variables are therefore integrated numerically in small steps over time, until the desired end time is reached. Consequently, efficient and accurate numerical integration methods are critical for many applications.
The computational effort for numerical integration is linked to the system size, and over time mathematical models have become increasingly detailed to achieve better predictions. Nevertheless, even models of moderate complexity result in numerical challenges when parameter sensitivities are needed. For instance, the parameter sensitivities can be integrated naively alongside the system variables, but this implies integrating a system of size n_{ x }×(1 + n_{ p }), where n_{ x } and n_{ p } are the number of system variables and system parameters respectively [3].
Additionally, the solution of a system of ODEs is often used in system identification processes where global optimization or probabilistic inference are required [4, 5]. In such cases, thousands, if not millions of trajectories need to be computed. Assessing the quality of the identified model, for instance with respect to the uncertainty in parameter values, again requires computing the local parameter sensitivities [6]. Although local sensitivity information can often help improve the overall estimation process, sensitivity computations are rarely included for performance reasons. Specific efficient methods exist for cases in which only scalar valued functionals are optimized [7] or oscillatory systems are considered [8]. Yet in many other cases, such as optimal control [9, 10], the identification of relevant parameters [11], model reduction and simplification [12] or parameter training [13], the full parameter sensitivities need to be computed. Consequently, improvements with respect to the speed with which the original ODE systems and their parameter sensitivities can be reliably integrated may affect the entire process significantly.
These issues are not new and they concern many application domains. Considerable efforts have been invested in establishing reliable and efficient generalpurpose ODE solvers for dynamic systems and—to a lesser extent—for the associated parameter sensitivities. Here, however, we are concerned with solving systems of ODEs as they typically occur in the simulation of biochemical reaction networks in systems biology [6]. We show that rather general characteristics of such systems allow for the development of application domainoriented ODE solvers with novel numerical features (with potential broader applicability), and with superior performance compared to stateoftheart, widely employed generalpurpose solvers. To provide some context for this claim, we first briefly review key characteristics of systems biology models in the form of ODEs, and general methods for the numerical integration of ODEs.
Dynamic models of (bio)chemical networks
When the effects of stochastic noise and of discrete molecule numbers are negligible, ODE systems can be used to describe chemical or biological reaction networks. Then_{ x } timedependent state variables x_{ i }(t,p), i = 1…n_{ x }, which represent the concentrations of the molecules of interest at time t and are usually known at some initial time t = t_{0}, evolve following
where f(x(t),p) is a system of functionsf_{ i }(x(t),p) modelling the conversion rate of each respective variable x_{ i }(t,p)at time t, and pis a vector of n_{ p }system parameters.
The local parameter sensitivities with respect to some parameter p_{ k } are defined as
which is the vector of the derivatives of all variables x_{ i }with respect to the parameter p_{ k }. Similar to the dynamics in Eq. (1), the parameter sensitivities’ time evolution follows a system of ODEs given by differentiating Eq. (2) with respect to t:
where J_{ f }(x(t)) is the Jacobian matrix of f(x(t)) with respect to x(t); note that we drop explicit dependencies on pto simplify notation. Initial conditions for Eq. (3) are set according to whether the initial conditions for the states in Eq. (1) depend on the parameters or not [3].
Consider, for example, the biochemical scheme of a MichaelisMenten type enzymatic reaction
where x_{1−4}correspond to enzyme, substrate, enzymesubstrate complex, and product concentrations, respectively. With massaction kinetics, the reaction network translates to the dynamic system
Such problems are often well solved by general purpose ODE solvers, but (bio)chemical reaction networks offer a number of features that may be exploited by more specialized solvers, resulting in faster and/or more precise simulations. For instance, in enzyme kinetics, reversible association and dissociation processes are usually much faster than product formation. The resulting stiffness severely limits the types of numerical methods that can be used for ODE integration.
An opportunity for increasing solver efficiency, however, presents itself because most (bio)chemical reaction networks are only weakly interconnected. More specifically, the change in every concentration x_{ i } usually depends on the concentration of very few other products. Poor connectivity is reflected in sparse Jacobians J_{ f }(x(t)), where nonzero elements correspond to interactions between components (that is, we have a correspondence to the network graph’s adjacency matrix). For the simple example Eq. (4),
with closed and open circles indicating nonzero and zero elements, respectively. Even in this dense subnetwork, the number of nonzeros \mathrm{nn}{z}_{J}=5/8{n}_{x}^{2} implies that we do not need to compute a substantial number of terms to determine the Jacobian.
Many largescale biological networks have a scalefree structure, that is, most of their nodes have few interactions, but a small number of hubs with many interactions exist [14]. This prevents an easy decomposition of a large network into subsystems that can be handled (and integrated) independently. Therefore, despite the sparsity of the Jacobian, model size remains a major issue for numerical performance.
Two more general aspects also need to be considered. Firstly, due to the growing use of abstract modeling software, the reactions and the underlying reaction equations are usually available to us as abstract models, such as smbl[15], which we can analyze and manipulate analytically. Secondly, since parametric uncertainty is abundant in biology, sensitivity analysis, i.e. the integration of the parameter sensitivities s_{ k }(t), requires particular attention. Hence, an ideal ODE solver for our application domain would efficiently and reliably handle large, stiff dynamic systems including their parameter sensitivities, and optimally exploit the systems’ nontrivial sparsity and analytic access.
Methods for ODE integration
Almost all ODE integrators work under the assumption that the change in each variable x_{ i }over time can be modeled using a polynomial in t. Consider the Taylor expansion of the variables x(t) around t = t_{0} to advance the system by a step of size h:
If the factors ∂^{k}x(t) / ∂ t^{k} / k!decrease sufficiently quickly and the higherorder terms become insignificant as of some degree n, then we can reliably approximate the new solution by a polynomial of degree n in h. In explicit integrators, previously computed values of x(t) and the derivatives ∂ x(t) / ∂t are used to construct a polynomial g_{ n }(t) of degree n and to extrapolate the value of x(t + h) ≈ g_{ n }(t + h). In implicit integrators, a solution x(t + h) is sought such that it matches that of a polynomial g_{ n }(t) of degree n interpolated through previous values of x(t) and/or their derivatives, and the derivative at the solution x(t + h)itself. In general, implicit integrators are more accurate for stiff ODEs, where the derivatives in Eq. (5) do not decay sufficiently quickly.
Within the two larger classes, different integrators are characterized by the amount of previous values of x(t) and their derivatives which they use to approximate x(t + h). Table 1 lists some common integration methods; see [16] for a comprehensive review.
Despite the commensurate degree of freedom in designing ODE integrators, and the number of algorithms for the numerical integration of ODEs that have been published over the past 40 years, only very few of them have found widespread application. Practical considerations—any method should be easily accessible to its end users, who are usually not interested in manipulating or even formulating the underlying equations themselves—are certainly major causes for this convergence [17]. However, a closer analysis of the most popular solvers for stiff ODE systems reveals another cause, namely incremental evolution.
In this area, the first major piece of software was the GEAR package [18], which by 1996 evolved into cvode[19], a part of the Sundials suite of nonlinear and differential/algebraic equation solvers [20]. The default integrators in Matlab (The MathWorks, Natick, MA) such as ode15s[21] employ similar integration rules and error estimates. Both the Sundials suite and Matlab are used increasingly in systems biology [22], but it is not evident that they are optimal for this application domain.
Methods
A secondderivative integrator
All ODE solvers mentioned above use only values of x(t)and \stackrel{\u0307}{\mathbf{x}}\left(t\right) to approximate x(t + h). Here, we differ from these methods in that we also employ the second derivatives:
(for notational simplicity, we will write J_{ f }(t)and f(t) instead of J_{ f }(x(t)) and f(x(t)), respectively). Note that this second derivative with respect to the time t should not be confused with the secondorder sensitivities described and used in [9, 10, 23], which are the second derivatives of the system variables with respect to the system parameters.
The use of second derivatives was first suggested in [24] and later studied in detail in [25], [26] and [27], and the resulting formulas were shown to have good stability properties. A more recent study [28] reinforces the stability and potential efficiency gains for stiff systems through secondderivative methods. However, despite several published implementations [27, 28], these methods have not yet found wide acceptance because, despite being Astable, they are only stable at infinity if only the second derivative at t + h is used [25] (see Section S3 in Additional file 1 and Additional file 2 for details).
The second derivatives in Eq. (6) may seem somewhat clumsy and expensive to evaluate since they require the construction of the Jacobian J_{ f }(t) and the evaluation of a matrixvector multiplication J_{ f }(t)f(t). Remember, however, that (bio)chemical reaction network models typically have sparse Jacobians. As a consequence, the cost of constructing J_{ f }(t) and of evaluating the product J_{ f }(t)f(t)grows only linearly with the number of variables and not quadratically, as the matrixvector product would imply. Furthermore, since we usually have an abstract representation of the governing equations, we can compute each entry of \stackrel{\u0308}{\mathbf{x}}\left(t\right) explicitly, much in the same way we evaluate the entries of f(t). For instance, the explicit second derivatives of the system Eq. (4) are:
In most cases, the evaluation of the second derivatives is not much more expensive than the evaluation of f(t).
For our secondderivative integrator, we construct an interpolating polynomial g_{4}(t) of degree n = 4 matching x(t)and the first and second derivatives at times t and t+h. This implicit method requires that we find x(t + h) such that
which, expanding g_{4}(t + h), gives us
where the righthand side is the polynomial through x(t), \stackrel{\u0307}{\mathbf{x}}\left(t\right), \stackrel{\u0308}{\mathbf{x}}\left(t\right), \stackrel{\u0307}{\mathbf{x}}(t+h) and \stackrel{\u0308}{\mathbf{x}}(t+h) evaluated at t+h (this is, incidentally, the original scheme proposed in [24]). The solution to this system of equations can be computed iteratively. More specifically, we start from an initial guess \stackrel{~}{\mathbf{x}}(t+h) that is computed with an explicit formula, and use a simplified Newton’s Method:
where M(t + h) is the Newton iteration matrix andJ_{ Jf }(t) is the Jacobian of Eq. (6) with respect to x(t):
The Jacobians {\stackrel{~}{\mathbf{J}}}_{f}(t+h) and {\stackrel{~}{\mathbf{J}}}_{\mathrm{Jf}}(t+h) are evaluated at the initial guess \stackrel{~}{\mathbf{x}}(t+h).
Using a secondderivative scheme, the evaluation of each Newton iteration is roughly twice as expensive as for firstderivative methods of the same degree since, in addition to f(t + h), we must also evaluateJ_{ f }(t + h)f(t + h). The advantage of this scheme, however, becomes obvious once we consider the truncation error. By replacing x(t + h) with the Taylor expansion around t, we obtain
for the truncation error of our secondderivative formula. For firstderivative methods of the same degree, assuming a constant step size h, this error is
in the case of the BDF and the AdamsMoulton formula of degree four, respectively. These truncation errors are 72 times and 19 times larger than the error of our secondderivative formula (assuming the fifth derivative x^{(5)}(t) is approximately constant in [t_{n−3},t_{n + 1}], see Section S2 and Figure S1A in Additional file 1 and Additional file 2 for details). The large difference stems from the dependence of the interpolation error on the width of the interpolation interval, e.g. for the BDF and the AdamsMoulton formula, this interval is four times larger.
Error estimates and step size adjustment
In any ODE integration scheme, the local error estimate and the stepsize adjustment are crucial to both its accuracy and its efficiency. The stepsize adjustment uses the error estimate of a previous integration step to predict the largest possible next step h satisfying the required tolerance. With imprecise error estimates, the stepsize adjustment has to be conservative to preserve accuracy, or it risks producing an imprecise result.
In most implicit ODE solvers, the local error is either estimated from the difference between the initial estimate \stackrel{~}{\mathbf{x}}(t+h), usually computed with an explicit rule, and the final converged step x(t + h), or as the difference between two rules of different degree over the previous x(t) and the converged step x(t + h). These approaches mainly consider computational efficiency because, ideally, to estimate the error of a formula of degree d_{1}, we need to compute a better approximation of degree d_{2} > d_{1}. The difference between both converged solutions x_{1}(t + h) and x_{2}(t + h) can then be used to approximate the difference between the lowerdegree estimate and the exact solution x^{⋆}(t + h). However, this requires two Newton iterations to compute both solutions and if both rules have different weights for the values of \stackrel{\u0307}{\mathbf{x}}(t+h) and \stackrel{\u0308}{\mathbf{x}}(t+h), we would need to invert or decompose two different matrices to compute a Newton iteration (Eq. (8)).
We propose a different approach that may better reconcile accuracy with computational cost. We first compute the converged lowerdegree solutionx_{1}(t + h)and use it as an initial estimate for the Newton iteration of the higherdegree solution. Since we are not actually interested in the exact solution x_{2}(t + h), but only in an approximation of the difference between the two solutions, it suffices to compute just one Newton step to get a firstoder approximation of that difference. Note that, in principle, this still requires the inversion or decomposition of a different matrix for the Newton iteration.
However, for our secondderivative solver, we can compute the second approximation as the polynomial g_{5}(t) that interpolates x(t) at the same nodes as g_{4}(t) plus the second to last node x(t−h_{−1}). In this case, the weights in the Newton iteration matrix are similar. If the current step size h and the previous step size h_{−1} are equal, the weights for J_{ f }(t + h) and J_{ Jf }(t + h) are 14/31 and 2/31, respectively, which is close to the values for g_{4}(t)of 1/2 and 1/12. We therefore reuse the Newton iteration matrix in Eq. (8) to compute the first approximation x_{1}(t + h) and obtain the local error estimate
Note that the estimate ε approximates the truncation error Eq. (10). Assuming that x^{(5)}(t)varies only slowly between two time steps, we can compute a scaling σ such that the error of the next step of size σh is equal to a prescribed tolerance τ:
Note that if the assumptions on x^{(5)}(t)do not hold, the error estimate in the next time step will fail, causing the step size to be reduced automatically. Furthermore, if we adjust h to fulfill the requested tolerance τ exactly, the error estimate will be larger than τapproximately half of the time. Therefore, in practice, we choose σ such that the next error will be τ/2. This gives us a recipe to adjust the step size from one integration step to the next and, hence, the last key ingredient of a functional secondderivative ODE solver (see Section S2 and Figure S1B of the Additional file 1 and Additional file 2 for details).
Parameter sensitivities
For n_{ x } variables and n_{ p }parameters, the naive approach to sensitivity calculation implies integrating a system of n_{ x }×(1 + n_{ p }) variables and, by consequence, inverting or decomposing matrices of that size within the Newton iteration. However, the system variables x(t)do not depend on the parameter sensitivities, yet the sensitivities depend on x(t). Hence, we can, in each step, first compute the values x(t + h) and, once they have converged, compute the s_{ k }(t + h)in a separate step using the same integration rule. This staggered approach was first introduced in Caracotsis & Stewart [29], then extended by Maly & Petzold [30], and finally implemented in the Sundials cvodes ODE solver, a modified version of cvode capable of sensitivity analysis [31].
To integrate the parameter sensitivities in our secondderivative solver in a similar way, we need to compute the second derivatives
The equation in the implicit step using the secondderivative rule in Eq. (7) for the parameter sensitivities s_{ k }(t) thus becomes
which, after isolating the sole unknown term s_{ k }(t + h)leads to
We then have two alternatives to compute s_{ k }(t + h): either iteratively using Newton’s method to solve Eq. (14), or directly by inverting or decomposing the matrix on the lefthand side of Eq. (15). The iterative approach via Eq. (14) is equivalent to the one suggested in [30], and we can reuse the inverted or decomposed iteration matrix used to compute the variables x(t + h)in Eq. (8). However, one has to reevaluate the Jacobians at each iteration to compute {\stackrel{\u0307}{\mathbf{s}}}_{k}\left(t\right) and {\stackrel{\u0308}{\mathbf{s}}}_{k}\left(t\right) because {\stackrel{~}{\mathbf{J}}}_{f}(t+h) and {\stackrel{~}{\mathbf{J}}}_{\mathrm{Jf}}(t+h) are evaluated at the initial estimate \stackrel{~}{\mathbf{x}}(t+h), and not at the converged solution x(t + h). To compute s_{ k }(t + h)directly, which corresponds to the original approach in [29], we need to recompute the Jacobians and the inverse or decomposition of the lefthand side of Eq. (15). For small n_{ x }, however, this extra matrix computation may be advantageous over running the Newton iteration for each parameter p_{ k }.
Furthermore, if the Jacobians do not vary significantly over time, they can be reused as the matrices {\stackrel{~}{\mathbf{J}}}_{f}\left(t\right) and {\stackrel{~}{\mathbf{J}}}_{\mathrm{Jf}}\left(t\right) for the Newton iteration of the next step. Such an approach offers an advantage if the cost of running an additional n_{ p } Newton iterations to compute the parameter sensitivities iteratively outweighs the cost incurred by the slower convergence due to using older Jacobians in the next step. In our secondderivative integrator, we therefore compute the parameter sensitivities directly as per Eq. (15).
Framework for conversion of SBML models
In order to generate the matrices J_{ f }(t) and J_{ Jf }(t), as well as the second derivative \stackrel{\u0308}{\mathbf{x}}\left(t\right) automatically, we established a framework that automatically translates arbitrary models from the standard SBML format [15] to Matlab functions or Clanguage code. The framework also generates routines to compute the parameter derivatives ∂ f(t)/∂ pand \partial \stackrel{\u0308}{\mathbf{x}}\left(t\right)/\partial \mathbf{p} necessary for the parameter sensitivity computations. This conversion, which needs to be done only once per model, exploits the sparsity of the corresponding matrices by generating compact expressions for their nonzero entries only, making them efficient to evaluate. It uses the Matlab Symbolic Toolbox to manipulate, differentiate and simplify the resulting expressions automatically (see Sections S1.3 and S1.4 in the Additional file 1 and Additional file 2 for details).
Results and discussion
Implementation and testing
We implemented the secondderivative ODE integrator as odeSD in Matlab and as odeSD _{ mex } in the C programming language, using the Matlab mex interface with calls to the LAPACK and BLAS libraries for the linear algebra operations. Both solvers provide an interface similar to that of the Matlab default integrators. Additionally, a native Clanguage version, odeSD _{ c }, was implemented for use outside of the Matlab programming environment. All implementations could operate on any type of ODEbased model, but the overall implementation is targeted to systems biology models in standard SBML format, for which we developed an automatic model conversion framework (see Methods). The implementation details are described in Sections S1.1 and S1.2 of the Additional file 1 and Additional file 2.
We compared our algorithm against three integrators which use Newton’s method to compute each implicit step:

1.Matlab’s
default integrator for stiff systems, ode15s[21], which uses a 5point Numerical Differentiation Formula (NDF), a more stable variant of the BDF integration rules; it is used as the default integrator in SBToolbox2 [22],

2.the
cvode integrator from the Sundials suite [20] which employs variableorder BDFs of up to degree 4; it is the integrator used in the SBML ODE Library (SOSlib) [32], and

3.the
radau5 integrator, a fifthorder threestage implicit RungeKutta method for stiff systems described in [33] and implemented in Matlab [34].
The Matlab interface supplied by the sundialsTB toolbox [35] served to run the Sundials integrators which are implemented in C.
For performance evaluation, we selected a number of curated systems biology models from the BioModels database [36] (Table 2). This set comprises systems of different sizes (up to the largest models available in the database) and characteristics, namely convergence to steadystate and (stiff) oscillatory behavior. Note that all models have sparse Jacobians, as is evident from the number of nonzero elements nnz(J_{ f }).
Integrator performance without parameter sensitivities
The results of the performance comparison without sensitivity analysis for a wide range of integration tolerances are summarized in Figure 1 (see Additional file 1: Figures S23 for details). The average computational times for our integrator were comparable (odeSD _{ mex }vs. cvode) to, or slightly lower (odeSD vs. ode15s or radau5) than those of the firstderivative solvers (Figure 1A), except for low numerical tolerances. Importantly, the secondderivative integrator required approximately half as many steps as ode15s or cvode (Figure 1B), despite these three integrators using rules of the same degree of precision. The radau5 integrator used less steps than odeSD, but it computes two additional intermediate steps per full step. The smaller number of steps in odeSD is due to a combination of both the smaller truncation error of the secondderivative rule and the better accuracy of the improved error estimate. For more detailed results and discussion, see Section S3 and Figure S2 of the the Additional file 1 and Additional file 2.
To assess the relative accuracy of odeSD, we compared the results of all models computed with different relative tolerances with an ‘accurate’ reference solution computed using radau5 with the relative tolerance set to 10^{−15}, analogously to the precision/work tests in [26]. The measured precision for each model and integrator is the maximum relative error in the final step for each state larger than machine precision in the reference solution. Figure 2 shows these results for all models in Table 2. These results are summarized in Figure 1E with the CPU time averaged over all models and the precision binned to the closest power of 10. Overall, without computing the parameter sensitivities, the new integrator is competitive, in terms of accuracy and efficiency, with highly optimized state of the art solvers for hard numerical problems in our application domain.
Integrator performance with parameter sensitivities
The performance comparison with sensitivity calculations requires two additional considerations: Since ode15s and radau5 do not provide any special functionality for computing parameter sensitivities, we used an augmented system of size n_{ x }×(n_{ p } + 1)including an analytic sparse Jacobian for each model, and the sensitivities s_{ k }(t), k=1…m were integrated alongside the system variables. cvodes, the sensitivity analysisenabled version of cvode from the sundials package, uses a simultaneous integrator based on the method of Maly & Petzold [47]. Optionally, the staggered integrator of Feehery et al.[30] can be selected, but this did not produce better results.
In all cases, parameter sensitivities were integrated to the same precision as the system variables. As with the integration without sensitivities, precision/work diagrams were computed for all models with sensitivities, omitting the cases in which ode15s failed completely.
As one detailed example, Figure 3A shows the computation times for a relative tolerance of 10^{−6}. In most cases, the compute times with sensitivities are substantial (see also Additional file 1: Figure S3 for other tolerances). For ode15s and radau5, the size of the augmented system quickly becomes a problem as the solution of the linear system of equations in the Newton iteration scales cubically with the number of variables. In terms of the additional effort for the sensitivity computation, we note that the secondderivative integrators are more efficient, often increasing the compute time only two or threefold, whereas the overhead is substantial for cvodes (Figure 3B). The results for cvodes are best explained if we keep in mind that whenever the righthand side f(·) is evaluated, the algorithm also computes {\stackrel{\u0307}{\mathbf{s}}}_{k}\left(t\right), k=1…n_{ p }, which, as per Eq. (3), requires an evaluation of the Jacobian J_{ f }(·). As a consequence, our secondderivative integrator outperforms the Sundials solver when implemented in C using the Matlab mex interface (odeSD _{ mex }), and even in native Matlab (odeSD) for all larger models. Note that the higher compute times for odeSD _{ mex }vs. odeSD in some cases are the result of a more refined handling of nearsingular matrices by Matlab in the latter.
In order to obtain results independent of any potential inefficiencies of the Matlab interface, the same performance analysis was run using odeSD _{ c }and cvodes with natively compiled Clanguage functions for the righthand sides. The results of this comparison are summarized in Figure 4 (see Additional file 1: Figure S4 in the Additional file 1 and Additional file 2 for the detailed precisionwork diagrams).
The higher efficiency of odeSD, odeSD _{ mex }and odeSD _{ c }holds also for averages over all models and for a wide range of numerical tolerances (Figure 3C). Except for highprecision integration, we achieve approximately two to threefold speedups. These general findings also hold when compute time is assessed as a function of numerical precision (Figure 5).
To explain the performance, consider that although odeSD and odeSD _{ mex } also evaluate the Jacobians in each step, both the much smaller number of steps required (Figure 3D), which is of no particular advantage when the parameter sensitivities are not computed, and the reuse of the Jacobians for sensitivity computations lead to significantly shorter execution times since substantially fewer evaluations of f(·) and of the Jacobians J_{(·)}(·)are needed (Figure 3D). Since the latter dominates the integration cost, all three versions of odeSD outperform cvodes in all but the smallest systems. These results are not a consequence of the sparsity of the systems per se, but of the more precise integration rule which can be computed efficiently thanks to sparsity. The better error estimate and the computation of local parametric sensitivities are therefore particular strengths of our ODE solver based on second derivatives.
Conclusions
We have presented an integrator for ODE systems resulting from the modeling of chemical and biological reaction networks, which are often stiff and sparse. For the realistic systems biology models tested, the new integrator outperforms commonly used state of the art integrators when parameter sensitivities are required. It is competitive in integrating the system equations alone, despite limitations for specific models near the steady state. The improvements with respect to sensitivity calculations are critical for many applications to drive highly computeintensive (global) optimization and estimation processes.
The improvements themselves are due to a combination of several factors: The more accurate secondderivative rule allows us, in combination with a better error estimate, to take larger steps, which in turn allows us to reduce the number of otherwise expensive sensitivity calculations. The reuse of the Jacobians from the sensitivity calculations further reduces the total computational costs. Although each integration step is more expensive than in firstderivative methods, due to the additional secondderivative information that needs to be computed, far less steps are required in total, resulting in a more efficient method.
To be of practical relevance for applications in systems biology, odeSD and odeSD _{ mex } are accessible via Matlab interfaces^{a}, and we plan to make them more easily available through integrated modeling environments such as the SBToolbox2 [22] and COPASI [48], e.g. via the native Clanguage interface. To accelerate larger optimization and estimation processes, further efficiency improvements through the use of sparse matrix routines and by more elaborate step size control schemes are possible.
In terms of numerical algorithms, to our knowledge, this is the first practical application of a secondderivative integration method with good performance. Key, novel features of our integrator, such as a more precise error estimator and direct computation of parameter sensitivities with reuse of the Jacobians, may well be suited for other problems or types of integrators. Importantly, while our integrator has been developed for (bio)chemical and reaction networks, it is still quite general in targeting stiff and sparse ODE systems. Overall, we feel that a lot is to be gained by adapting general algorithms to specific problem domains, and that results from the work on specific problems can spill over to the broader field.
Availability
The integrator (Matlab and Clanguage versions) and the model conversion framework are available via http://www.csb.ethz.ch/tools.
Endnotes
^{a}We note that it is not possible to execute odeSD _{ mex }in the 64bit Windows version of Matlab R2010a as well as in 64bit Linux versions prior to R2010a in our testing environments, for reasons of memory allocation problems in Matlab.
References
Chen WW, Niepel M, Sorger PK: Classic and contemporary approaches to modeling biochemical reactions. Genes Dev 2010,24(17):18611875. http://dx.doi.org/10.1101/gad.1945410 10.1101/gad.1945410
Gutenkunst RN, Waterfall JJ, Casey FP, Brown KS, Myers CR, Sethna JP: Universally sloppy parameter sensitivities in systems biology models. PLoS Comput Biol 2007,3(10):18711878. http://dx.doi.org/10.1371/journal.pcbi.0030189
Turányi T, Rabitz H: Local methods. In Sensitivity analysis. Edited by: Saltelli A, Chan K, Scott E. Chichester, UK: John Wiley & Sons, Ltd.; 2000:81100.
Banga JR, BalsaCanto E: Parameter estimation and optimal experimental design. Essays in Biochem: Syst Biol 2008, 45: 195209.
Cheng H, Sandu A: Efficient uncertainty quantification with the polynomial chaos method for stiff systems. Math Comput Simul 2009,79(11):32783295. 10.1016/j.matcom.2009.05.002
Doyle FJ, Stelling J: Systems interface biology. J R Soc Interface 2006,3(10):603616. 10.1098/rsif.2006.0143
Cao Y, Li S, Petzold L, Serban R: Adjoint Sensitivity Analysis for DifferentialAlgebraic Equations: The Adjoint DAE System and Its Numerical Solution. SIAM J Sci Comput 2003,24(3):10761089. 10.1137/S1064827501380630
Wilkins AK, Tidor B, White J, Barton PI: Sensitivity Analysis for Oscillating Dynamical Systems. SIAM J Sci Comput 2009,31(4):27062732. 10.1137/070707129
Vassiliadis VS, Canto EB, Banga JR: Secondorder sensitivities of general dynamic systems with application to optimal control problems. Chem Eng Sci 1999,54(17):38513860. 10.1016/S00092509(98)004321
BalsaCanto E, Banga JR, Alonso AA, Vassiliadis VS: Efficient Optimal Control of Bioprocesses Using SecondOrder Information. Ind Eng Chem Res 2000,39(11):42874295. 10.1021/ie990658p
Sher A, Wang K, Wathen A, Mirams G, Abramson D, Gavaghan D: A Local Sensitivity Analysis Method for Developing Biological Models with Identifiable Parameters: Application to Ltype Calcium Channel Modelling. IEEE Sixth International Conference on eScience 2010, 176181.
Liu G, Swihart MT, Neelamegham S: Sensitivity, principal component and flux analysis applied to signal transduction: the case of epidermal growth factor mediated signaling. Bioinformatics 2005,21(7):11941202. 10.1093/bioinformatics/bti118
Chen WW, Schoeberl B, Jasper PJ, Niepel M, Nielsen UB, Lauffenburger DA, Sorger PK: Inputoutput behavior of ErbB signaling pathways as revealed by a mass action model trained against dynamic data. Mol Syst Biol 2009.,5(239):
Barabási AL, Oltvai Z: Network biology: understanding the cell’s functional organization. Nat Rev Genet 2004, 5: 10113. 10.1038/nrg1272
Hucka M, Finney A, Sauro H, Bolouri H, Doyle J, Kitano H, Arkin A, Bornstein B, Bray D, CornishBowden A, Cuellar A, Dronov S, Gilles E, Ginkel M, Gor V, Goryanin I, Hedley W, Hodgman T, Hofmeyr J, Hunter P, Juty N, Kasberger J, Kremling A, Kummer U, Novere NL, Loew L, Lucio D, Mendes P, Minch E, Mjolsness E, Nakayama Y, Nelson M, Nielsen P, Sakurada T, Schaff J, Shapiro B, shimizu T, Spence H, Stelling J, Takahashi K, Tomita M, Wagner J, Wang J: The Systems Biology Markup Language (SBML): a medium for representation and exchange of biochemical network models. Bioinformatics 2003,19(4):52431. 10.1093/bioinformatics/btg015
Gupta GK, SacksDavis R, Tischer PE: A Review of Recent Developments in Solving ODEs. ACM Comput Surv 1985, 17: 547. 10.1145/4078.4079
Cash JR: Efficient numerical methods for the solution of stiff initialvalue problems and differential algebraic equations. Proc R Soc London Series aMath Phys Eng Sci 2002,459(2032):797815.
Hindmarsh AC: GEAR: Ordinary Differential Equation System Solver.. Technical Report UCID30001, Rev. 3, Lawrence Livermore National Laboratory 1974
Cohen SD, Hindmarsh AC: CVODE, a stiff/nonstiff ODE solver in C. Comput Phys 1996,10(2):138143.
Hindmarsh AC, Brown PN, Grant KE, Lee SL, Shumaker DE, S WC, R S: SUNDIALS: Suite of Nonlinear and Differential/Algebraic Equation Solvers. ACM Trans on Math Software 2005,31(3):363396. 10.1145/1089014.1089020
Shampine LF, Reichelt MW: The MATLAB ODE Suite. SIAM J Sci Comput 1997, 18: 122. 10.1137/S1064827594276424
Schmidt H, Jirstrand M: Systems Biology Toolbox for MATLAB: a computational platform for research in systems biology. Bioinformatics 2006,22(4):5145. [Schmidt, Henning Jirstrand, Mats Research Support, NonU.S. Gov’t England Bioinformatics (Oxford, England) Bioinformatics. 2006 Feb 15;22(4):5145. Epub 2005 Nov 29.]. 10.1093/bioinformatics/bti799
Özyurt DB, Barton PI: Cheap second order directional derivatives of stiff ODE embedded functionals. SIAM J Sci Comput 2005,26(5):17251743. 10.1137/030601582
Obrechkoff N: Sur les quadrature mecaniques. Spisanic Bulgar Akad Nauk 1942, 65: 191289. [Reviewed in Mat. Rev. 10:70]
Enright WH: Second derivative multistep methods for stiff ordinary differential equations. SIAM J Numer Anal 1974,11(2):321331. 10.1137/0711029
Hairer E, Norsett S, Wanner G: Solving Ordinary Differential Equations I. Berlin: Springer Verlag; 1987.
Addison CA, Gladwell I: Second derivative methods applied to implicit first and secondorder systems. Int J Numer Methods Eng 1984,20(7):12111231. 10.1002/nme.1620200704
Corliss G, Griewank A, Henneberger P, Kirlinger G, Potra F, Stetter H: Highorder stiff ODE solvers via automatic differentiation and rational prediction. In Numerical Analysis and Its Applications, Volume 1196 of Lecture Notes in Computer Science. Edited by: Vulkov L, Wasniewski J, Yalamov P. Berlin /Heidelberg; 1997:114125.
Caracotsios M, Stewart WE: Sensitivity analysis of initial value problems with mixed ODEs and algebraic equations. Comput Chem Eng 1985,9(4):359365. 10.1016/00981354(85)850146
Feehery WF, Tolsma JE, Barton PI: Efficient sensitivity analysis of largescale differentialalgebraic systems. Appl Numer Math 1997, 25: 4154. 10.1016/S01689274(97)000500
Serban R, Hindmarsh AC: CVODES, the sensitivityenabled ODE solver in SUNDIALS. Proceedings of the ASME International Design Engineering Technical Conferences and Computers and Information in Engineering Conference, Vol 6, Pts AC 2005, 257269.
Machné R, Finney A, Müller S, Lu J, Widder S, Flamm C: The SBML ODE Solver Library: a native API for symbolic and fast numerical analysis of reaction networks. Bioinformatics 2006,22(11):14061407. 10.1093/bioinformatics/btl086
Hairer E, Wanner G: Solving Ordinary Differential Equations II: Stiff and DifferentialAlgebraic Problems. Berlin: Springer Verlag; 1991.
Engstler C: Matlab implementation of the Radau IIA method of order5 by Ch. Engstler after the Fortran Code RADAU5 of Hairer/Wanner. 1999.http://na.unituebingen.de/projects.shtml
Serban R: sundialsTB v2.4.0, a Matlab interface to Sundials.. 2009. Technical Report UCRLSM212121, Lawrence Livermore National Laboratory
Li C, Donizelli M, Rodriguez N, Dharuri H, Endler L, Chelliah V, Li L, He E, Henry A, Stefan MI, Snoep JL, Hucka M, Novère NL, Laibe C: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. BMC Syst Biol 2010, 4: 92. http://dx.doi.org/10.1186/17520509492 10.1186/17520509492
Hornberg JJ, Bruggeman FJ, Binder B, Geest CR, de Vaate AJMB, Lankelma J, Heinrich R, Westerhoff HV: Principles behind the multifarious control of signal transduction. ERK phosphorylation and kinase/phosphatase control. FEBS J 2005, 272: 244258.
Kholodenko BN, Demin OV, Moehren G, Hoek JB: Quantification of short term signaling by the epidermal growth factor receptor. J Biol Chem 1999,274(42):3016930181. 10.1074/jbc.274.42.30169
Singh A, Jayaraman A, Hahn J: Modeling regulatory mechanisms in IL6 signal transduction in hepatocytes. Biotechnol Bioeng 2006,95(5):850862. 10.1002/bit.21026
Borisov N, Aksamitiene E, Kiyatkin A, Legewie S, Berkhout J, Maiwald T, Kaimachnikov NP, Timmer J, Hoek JB, Kholodenko BN: Systemslevel interactions between insulinEGF networks amplify mitogenic signaling. Mol Syst Biol 2009, 5: 256.
Ung CY, Li H, Ma XH, Jia J, Li BW, Low BC, Chen YZ: Simulation of the regulation of EGFR endocytosis and EGFRERK signaling by endophilinmediated RhoAEGFR crosstalk. FEBS Lett 2008,582(15):22832290. 10.1016/j.febslet.2008.05.026
Elowitz MB, Leibler S: A synthetic oscillatory network of transcriptional regulators. Nature 2000,403(6767):335338. 10.1038/35002125
Leloup Goldbeter: Chaos and birhythmicity in a model for circadian oscillations of the PER and TIM proteins in Drosophila. J Theor Biol 1999,198(3):445459. 10.1006/jtbi.1999.0924
Wolf J, Sohn H, Heinrich R, Kuriyama H: Mathematical analysis of a mechanism for autonomous metabolic oscillations in continuous culture of Saccharomyces cerevisiae. FEBS Lett 2001,499(3):230234. 10.1016/S00145793(01)025625
Goldbeter A, Pourquié O: Modeling the segmentation clock as a network of coupled oscillations in the Notch, Wnt and FGF signaling pathways. J Theor Biol 2008,252(3):574585. 10.1016/j.jtbi.2008.01.006
Xie Z, Kulasiri D: Modelling of circadian rhythms in Drosophila incorporating the interlocked PER/TIM and VRI/PDP1 feedback loops. J Theor Biol 2007,245(2):290304. 10.1016/j.jtbi.2006.10.028
Maly T, Petzold LR: Numerical methods and software for sensitivity analysis of differentialalgebraic systems. Appl Numer Math 1996,20(12):5779. 10.1016/01689274(95)001174
Mendes P, Hoops S, Sahle S, Gauges R, Dada J, Kummer U: Computational modeling of biochemical networks using COPASI. Methods Mol Biol 2009, 500: 1759. http://dx.doi.org/10.1007/9781597455251_2 10.1007/9781597455251_2
Acknowledgements
We thank Ernst Hairer for comments and discussions. This work was supported by the EU FP6 project BaSysBio (LSHGCT2006037469), the EU FP7 project UniCellSys (HEALTHF42008201142), the Swiss Initiative for Systems Biology SystemsX.ch (RTD project YeastX), by an ETH Excellence Scholarship (LW), and by Swiss National Science Foundation’s Individual Support Fellowships Nr. PBEZP2127959 and Nr. PA00P2134146 (PG).
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PG developed the secondderivative integration scheme for the parameter sensitivities and the new error estimate, as well as the final Matlab, mex and Clanguage versions. SD developed the automatic generation of the righthand sides. LW implemented and tested the first versions of the integrator. JS designed and conducted the experiments. Both PG and JS wrote the manuscript, which was read and approved by all authors.
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Gonnet, P., Dimopoulos, S., Widmer, L. et al. A specialized ODE integrator for the efficient computation of parameter sensitivities. BMC Syst Biol 6, 46 (2012). https://doi.org/10.1186/17520509646
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DOI: https://doi.org/10.1186/17520509646
Keywords
 Dynamical models
 ordinary differential equations
 parameter sensitivities
 integration