# Statistical ensemble analysis for simulating extrinsic noise-driven response in NF-κB signaling networks

- Jaewook Joo
^{1}Email author, - Steven J Plimpton
^{2}and - Jean-Loup Faulon
^{3}

**7**:45

https://doi.org/10.1186/1752-0509-7-45

© Joo et al.; licensee BioMed Central Ltd. 2013

**Received: **20 November 2012

**Accepted: **7 May 2013

**Published: **7 June 2013

## Abstract

### Background

Gene expression profiles and protein dynamics in single cells have a large cell-to-cell variability due to intracellular noise. Intracellular fluctuations originate from two sources: *intrinsic* noise due to the probabilistic nature of biochemical reactions and *extrinsic* noise due to randomized interactions of the cell with other cellular systems or its environment. Presently, there is no systematic parameterization and modeling scheme to simulate cellular response at the single cell level in the presence of extrinsic noise.

### Results

In this paper, we propose a novel statistical ensemble method to simulate the distribution of heterogeneous cellular responses in single cells. We capture the effects of extrinsic noise by randomizing values of the model parameters. In this context, a statistical ensemble is a large number of system replicates, each with randomly sampled model parameters from biologically feasible intervals. We apply this statistical ensemble approach to the well-studied NF-κB signaling system. We predict several characteristic dynamic features of NF-κB response distributions; one of them is the dosage-dependent distribution of the first translocation time of NF-κB.

### Conclusion

The distributions of heterogeneous cellular responses that our statistical ensemble formulation generates reveal the effect of different cellular conditions, e.g., effects due to wild type versus mutant cells or between different dosages of external stimulants. Distributions generated in the presence of extrinsic noise yield valuable insight into underlying regulatory mechanisms, which are sometimes otherwise hidden.

### Keywords

Statistical ensemble Extrinsic noise Cell to cell variability NF-κB signal transduction network## Background

Single cell imaging generated a surge of interest in the intracellular dynamics of biochemical species, uncovering significant cell-to-cell variations in gene expression [1–8] and protein dynamics [9, 10]. This variability originates from intrinsic [1–8] and extrinsic noise [3, 6, 10] and critically affects cellular decision-making processes [9–13]. Moreover, cellular response averaged over a population of cells is oftentimes noticeably different from the responses of single cells. The variability in the latter contains rich information regarding the regulatory mechanisms in operation. Here, we present a novel computational method to predict the distribution of extrinsic noise-driven heterogeneous cellular responses and to unravel discrepancies between single-cell versus population-averaged responses.

*Intrinsic*noise refers to the pure probabilistic nature of individual biochemical reactions occurring within a cell. When the number of intracellular constituents is large, the cell’s behavior is well approximated by its expectation value according to the law of large numbers. But at the single-cell level, the number of molecules of certain species critical to a particular biochemical pathway can be small, and the range of statistical variation in the system needs to be considered [1–8].

*Extrinsic*noise refers to random interactions of the cell with other cells or its environment. Extrinsic fluctuations can originate from cells undergoing different stages of their cell cycle [15], fluctuations in the number of transcriptional regulators upstream of the signaling pathway of interest [3, 6, 9, 10], and cell-to-cell variability in the copy number of proteins inherited from parent cells during cell division [10]. Extrinsic noise can affect the dynamics of cellular constituents locally in a specific signaling pathway or globally over the entire cell. In Figure 1, we summarize the effects of intrinsic and extrinsic fluctuations in the NF-κB signaling networks. The full effect of extrinsic noise should include “all” external stochastic effects that influence the cell, particularly the temporal fluctuations in the cellular kinetic conditions. However, in Ref. [10], Spencer et al. identified the most important source of extrinsic noise as the protein copy number inherited from the parent cell during cell division. Large cell-to-cell variations in the copy number of enzyme and regulatory protein could randomize the likelihood and the speed of any intracellular biochemical reaction. This means we can effectively “lump” all the effects of protein copy number variations into variations in kinetic rate constants. This is an attractive approach, because rate constants are an input into a variety of biochemical pathway modeling techniques.

A pathway modeling framework that uses deterministic or stochastic differential equation models requires *a priori* knowledge of the structure of the biochemical reaction network, mathematical functional forms for the biochemical reactions, and associated reaction rate constants. Since limited or incomplete information is often all that is available to modelers, a computational model is often parameterized by using a nonlinear fitting algorithm. A conventional parameterization scheme identifies a single set of kinetic parameter values by minimizing the χ^{2} distance between experimental data and a prediction made by the model. Sloppy Cell and other similar parameterization algorithms include experimental errors in the parameterization by fitting to a rather large experimental error bar [16]. But both conventional and Sloppy Cell parameterization schemes assume a deterministic and homogeneous biological response to a stimulus and aren’t designed to handle the heterogeneous, stochastic behavior of single cells and its dependence on extrinsic noise.

In order to capture extrinsic noise and its effect on intracellular response, we propose a novel parameterization method, the “statistical ensemble” (SE) scheme, named after a key concept in statistical physics [17]. A cell is regarded as a complex system comprising a large number of components and elementary interactions among them. A population of cells consists of a large number of replicates, each with different microscopic intracellular states. The statistical ensemble average, or macroscopic observable, is equated with the cellular response averaged over the population of cells. The ensemble is generated by assigning randomly sampled values of kinetic rate constants and copy numbers of regulatory proteins to each cell in the ensemble. All other external noisy systems that interact with the cell, but which are not modeled explicitly, are treated as extrinsic noise. The effect of the noise is included in the sampling that produces the randomized microscopic state of each cell in the ensemble.

A key point is that the resulting dynamic response of the ensemble of cells is no longer a single output but is a distribution of heterogeneous responses. Each response can be computed independently, which allows for parallelism in the computation. An equal weight is assigned to the response from each replicate, to calculate the ensemble averaged cellular response. The SE scheme thus enables modeling of the irregular, dissimilar, and diverse individual cellular behaviors while reproducing the macroscopically observable population-level response.

In the most general sense, the success of the SE scheme depends on identifying and characterizing the biologically correct distribution of extrinsic noise for the system of interest, so that its effects can be encoded in the random sampling of cellular microstates. In this work, we use experimental population-level data to parameterize the range of feasible kinetic rate constants and copy numbers of specific molecules, and then sample uniformly around the mid-point of the range to generate cellular microstates. We illustrate the power of even this simplified SE approach for modeling the NF-кB signaling system.

NF-кB is a pleiotropic regulator of gene control and plays significant roles in various cellular functions such as differentiation of immune cells, development of lymphoid organs, and immune activation [18–20]. NF-кB shuttling between the nucleus and cytoplasm is auto-regulated by the NF-кB signaling module, which consists of IкB (inhibitor кB), IKK (IкB kinase), and NF-кB. In the absence of stimulus, IкB forms a hetero-dimeric complex with NF-кB, preventing NF-кB from entering into the nucleus. Upon stimulation, phosphorylated IKK catalyses the degradation of IкB from the IкB-NF-кB complex and frees up NF-кB whose nuclear localization initiates transcription of NF-кB target genes such as inflammatory cytokines (TNFα, IL-1, IL-6), chemotactic cytokines (MIP-1a), Th1 and Th2 response activation (IFN and IL-10), and lastly, but most importantly, negative regulators (IкBα, IкBβ, IкBϵ, and A20) which terminate the NF-кB signaling. Based on current knowledge of NF-кB signaling, Hoffmann *et al.* constructed a complex biochemical reaction network for the NF-кB signaling pathway consisting of IKK, NF-кB, and three IкB isoforms and transformed it into a set of ordinary differential equations with dozens of unknown kinetic parameter values [21]. After identifying a single set of parameter values yielding the best fit of the model to population level experimental data, they used their model to elucidate the role of each of three IкB isoforms: IкBα induces oscillatory shuttling of NF-кB while IкBβ and IкBϵ damp the oscillations [21]. Lipniacki *et al.* extended the model, showing that an additional negative regulator A20 has a definitive role as a NF-кB signal terminator, by deactivating IKK phosphorylation [22–24]. Using fluorescence microscopy, Nelson *et al.* and several other groups showed a remarkably heterogeneous intracellular response for this signaling network at the single-cell level; some cells exhibited sustained oscillatory shuttling of NF-кB while others exhibited non-oscillatory behavior [25–33].

## Results

### Statistical ensemble average of key biochemical species concentrations in the NF-кB signaling network is fit to experimental population-level data

#### The wild type case

#### The mutant case - double knocked-out IкB isoforms and knocked-out A20

#### Dependence of SE average on heterogeneity

In this subsection, we showed how the SE method with its many replicates is a model for a population of cells in a heterogeneous set of intracellular states. By varying the heterogeneity factor χ for sampling kinetic parameters used as inputs to the pathway model, fits to experimental data can be produced even when population-averaged data and single-cell data exhibit different characteristics, as in the NF-кB signaling system. In the next subsection, we discuss the distribution of single-cell NF-кB responses in more detail.

### Prediction of distributions of individual cellular responses for the wild type and mutants

#### Distributions of dynamic features

**Biochemical reactions and their associated reaction rate constants in the computational model of the NF-κB signaling network**

Reactions | I | II | III | IV | V |
---|---|---|---|---|---|

IKKa + IкBα → IKKa:IкBα | Aα | [a] | 0.2 | [1] | 0.1813 |

IKKa + IкBβ → IKKa:IкBβ | Aβ | [a] | 0.05 | [3] | 0.02997 |

IKKa + IкBϵ → IKKa:IкBϵ | Aϵ | [a] | 0.05 | [3] | 0.04244 |

IKKa + IkBα:NF-кB → IKKa:IкBα:NF-кB | Bα | [a] | 1 | [1] | 1.024 |

IKKa + IkBβ:NF-кB → IKKa:IкBβ:NF-кB | Bβ | [a] | 0.25 | [3] | 0.3683 |

IKKa + IkBϵ:NF-кB → IKKa:IкBϵ:NF-кB | Bϵ | [a] | 0.25 | [3] | 0.42 |

NF-кBn → NF-кBn + A20t | C1 | [b] | 0.0000005 | [1] | 0.000000506 |

0 → A20t | C2 | [c] | 0 | [1] | 0 |

A20t → 0 | C3 | [b] | 0.0004 | [1] | 0.0002438 |

A20t → A20t + A20 | C4 | [b] | 0.5 | [1] | 0.5807 |

A20 → 0 | C5 | [b] | 0.0003 | [1] | 0.0003769 |

IKKa:IкBα → IKKa + IкBα | Dα | [b] | 0.00125 | [2] | 0.002046 |

IKKa:IкBβ → IKKa + IкBβ | Dβ | [b] | 0.00175 | [2] | 0.0005609 |

IKKa:IкBϵ → IKKa + IкBϵ | Dϵ | [b] | 0.00175 | [2] | 0.002142 |

IKKa:IкBα:NF-кB → IKKa + IккBα:NF-кB | Dα | [b] | 0.00125 | [2] | 0.002046 |

IKKa:IkBβ:NF-кB → IKKa + IкBβ:NF-кB | Dβ | [b] | 0.00175 | [2] | 0.000561 |

IKKa:IкBϵ:NF-кB → IKKa + IкBϵ:NF-кB | Dϵ | [b] | 0.00175 | [2] | 0.002142 |

IKKa:IкBα:NF-кB → IKKa:IкBα + NF-кB | Eα | [b] | 0.000001 | [2] | 0.00000144 |

IKKa:IкBβ:NF-кB → IKKa:IкBβ + NF-кB | Eβ | [b] | 0.000001 | [2] | 0.00000124 |

IKKa:IкBϵ:NF-кB → IKKa:IкBϵ + NF-кB | Eϵ | [b] | 0.000001 | [2] | 0.00000064 |

IKKa:IкBα + NF-кB → IKKa:IкBα:NF-кB | Fα | [a] | 0.5 | [2] | 0.3789 |

IKKa:IкBβ + NF-кB → IKKa:IкBβ:NF-кB | Fβ | [a] | 0.5 | [2] | 0.2135 |

IKKa:IкBϵ + NF-кB → IKKa:IкBϵ:NF-кB | Fϵ | [a] | 0.5 | [2] | 0.3528 |

IкBα:NF-кB → NF-кB + IкBα | Gα | [b] | 0.000001 | [2] | 0.00000064 |

IкBβ:NF-кB → NF-кB + IкBβ | Gβ | [b] | 0.000001 | [2] | 0.00000044 |

IкBϵ:NF-кB → NF-кB + IкBϵ | Gϵ | [b] | 0.000001 | [2] | 0.00000069 |

IкBαn:NF-кBn → NF-кBn + IкBαn | Gα | [b] | 0.000001 | [2] | 0.00000064 |

IкBβn:NF-кBn → NF-кBn + IкBβn | Gβ | [b] | 0.000001 | [2] | 0.00000044 |

IкBϵn:NF-кBn → NF-кBn + IкBϵn | Gϵ | [b] | 0.000001 | [2] | 0.00000069 |

IкBα + NF-кB → IкBα:NF-кB | Hα | [a] | 0.5 | [2] | 0.4593 |

IкBβ + NF-кB → IкBβ:NF-кB | Hβ | [a] | 0.5 | [2] | 0.7753 |

IкBϵ + NF-кB → IкBϵ:NF-кB | Hϵ | [a] | 0.5 | [2] | 0.2895 |

IкBαn + NF-кBn → IкBαn:NF-кBn | Hα | [a] | 0.5 | [2] | 0.4593 |

IкBβn + NF-кBn → IкBβn:NF-кBn | Hβ | [a] | 0.5 | [2] | 0.7753 |

IкBϵn + NF-кBn → IкBϵn:NF-кBn | Hϵ | [a] | 0.5 | [2] | 0.2895 |

NF-кB → NF-кBn | I1 | [b] | 0.0025 | [1] | 0.003037 |

NF-кBn → NF-кB | K01 | [b] | 0.00005 | [3] | 0.00005537 |

IKKn → IKKa | K1 | [b] | 0.0025 | [1] | 0.003273 |

A20 + IKKa → A20 + IKKi | K2 | [a] | 0.1 | [1] | 0.07075 |

IKKa → IKKi | K3 | [b] | 0.0015 | [1] | 0.00202 |

0 → IKKn | Kprod | [c] | 0.000025 | [1] | 0.000009752 |

IKKn, IKKa, or IKKi → 0 | Kdeg | [b] | 0.000125 | [1] | 0.0001561 |

Volume ratio of cytoplasm to nucleus | Kv | 1 | 5 | [1] | 5 |

IкBαn:NF-кBn → IкBα:NF-кB | Lα | [b] | 0.01 | [1] | 0.013979 |

IкBβn:NF-кBn → IкBβ:NF-кB | Lβ | [b] | 0.005 | [3] | 0.001567 |

IкBϵn:NF-кBn → IкBϵ:NF-кB | Lϵ | [b] | 0.005 | [3] | 0.006583 |

IкBα:NF-кB → NF-кB | Mα | [b] | 0.000025 | [1] | 0.00002837 |

IкBβ:NF-кB → NF-кB | Mβ | [b] | 0.000025 | [3] | 0.00003609 |

IкBϵ:NF-кB → NF-кB | Mϵ | [b] | 0.000025 | [3] | 0.00000866 |

Total NF-кB concentration | NF-кB | [d] | 0.06 | [1] | 0.06 |

IKKa:IкBα:NF-кB → IKKa + NF-кB | Pα | [b] | 0.1 | [1] | 0.12928 |

IKKa:IкBβ:NF-кB → IKKa + NF-кB | Pβ | [b] | 0.05 | [3] | 0.06454 |

IKKa:IкBϵ:NF-кB → IKKa + NF-кB | Pϵ | [b] | 0.05 | [3] | 0.08434 |

IкBαn → IкBα | Qα | [b] | 0.0005 | [1] | 0.0005123 |

IкBβn → IкBβ | Qβ | [b] | 0.0005 | [3] | 0.0007398 |

IkBϵn → IkBϵ | Qϵ | [b] | 0.0005 | [3] | 0.0002184 |

IKKa:IкBα → IKKa | Rα | [b] | 0.1 | [1] | 0.123 |

IKKa:IкBβ → IKKa | Rβ | [b] | 0.1 | [3] | 0.03837 |

IKKa:IкBϵ → IKKa | Rϵ | [b] | 0.1 | [3] | 0.1571 |

IкBαn:NF-кBn → NF-кBn | Sα | [b] | 0.000001 | [2] | 0.00000037 |

IкBβn:NF-кBn → NF-кBn | Sβ | [b] | 0.000001 | [2] | 0.000001131 |

IкBϵn:NF-кBn → NF-кBn | Sϵ | [b] | 0.000001 | [2] | 0.000001037 |

NF-кBn → NF-кBn + IкBαt | Uα | [b] | 0.0000005 | [1] | 0.000000279 |

NF-кBn → NF-кBn + IкBβt | Uβ | [b] | 0 | [2] | 0 |

NF-кBn → NF-кBn + IкBϵt | Uϵ | [b] | 0.00000005 | [3] | 0.000000059 |

IкBα → IкBαn | Vα | [b] | 0.001 | [1] | 0.0009786 |

IкBβ → IкBβn | Vβ | [b] | 0.001 | [3] | 0.0004871 |

IkBϵ → IkBϵn | Vϵ | [b] | 0.001 | [3] | 0.00147 |

IкBα, IкBαn → 0 | Wα | [b] | 0.0001 | [1] | 0.000132 |

IкBβ, IкBβn → 0 | Wβ | [b] | 0.0001 | [3] | 0.000133 |

IкBϵ, IкBϵn → 0 | Wϵ | [b] | 0.0001 | [3] | 0.000042 |

IкBαt → IkBαt + IkBα | Xα | [b] | 0.5 | [1] | 0.4552 |

IкBβt → IкBαt + IкBβ | Xβ | [b] | 0.5 | [3] | 0.3828 |

IкBϵt → IкBαt + IкBϵ | Xϵ | [b] | 0.5 | [3] | 0.3304 |

0 → IкBαt | Yα | [c] | 0.00000005 | [3] | 0.000000084 |

0 → IкBβt | Yβ | [c] | 0.000000005 | [3] | 0.00000000414 |

0 → IкBϵt | Yϵ | [c] | 0.000000005 | [3] | 0.00000000508 |

IкBαt → 0 | Zα | [b] | 0.0004 | [1] | 0.0003375 |

IкBβt → 0 | Zβ | [b] | 0.0004 | [3] | 0.0002031 |

IкBϵt → 0 | Zϵ | [b] | 0.0004 | [3] | 0.0004742 |

#### Distribution of dynamic patterns

The individual time-series of the nuclear NF-кB concentrations can be classified into one of four dynamic patterns (damped oscillation, sustained oscillation, single peaked, and monotonic-increasing patterns) as shown in Figure 6. The underlying mechanism for each dynamic pattern is rather simple. The monotonic-increasing (or over-damped) pattern originates from strong negative feedback loops, while the single-peaked pattern results from weak negative feedback loops. The oscillatory patterns arise from intermediate-strength negative feedback loops. But it remains an open question to correlate each dynamic pattern with a specific cellular physiology [37–39]. To elucidate this connection, we stimulate the ensemble of NF-кB signaling networks with the same signal strength (TR = 1), for both the wild type and mutants. We then classify a thousand individual temporal profiles into one of the four dynamic patterns. The distributions of the patterns are represented by bar graphs in Figure 6 which shows that both the wild type and mutants exhibit at least two different patterns of response under the same strength of stimulation. For the wild type, most of the nuclear NF-кB profiles have a damped-oscillatory pattern, with less than 10% of the profiles as sustained-oscillatory. This indicates a damped-oscillatory response is the most probable, and it is robust against perturbation of the network parameter values. For the mutant with a knocked-out A20 gene, both single-peaked and damped-oscillatory patterns are nearly equally probable. But the damped oscillatory profiles are very similar to a single-peaked pattern. Thus for this mutant, a damped-oscillatory response occurs in a region of the parameter space where the negative regulators are not strong enough to induce the oscillatory pattern. For the mutant with IкBβ and IкBϵ genes double knocked-out, sustained-oscillatory and damped-oscillatory patterns are equally probable responses. The damped-oscillatory responses in this mutant are very different from those in the mutant with a knocked-out A20 gene, and are more similar to a sustained oscillation. The fraction of sustained-oscillatory responses (about 50%) dramatically increases in comparison to the wild type case (less than 10%). For mutants with IкBα and IкBβ genes double knocked-out and with IкBα and IкBϵ genes double knocked-out, their respective distributions are similar to that of the mutant with the A20 gene knocked-out. As shown in Figures 5(B) and 5(C) and Figure 4(B), both the individual profiles and the statistical ensemble average of the nuclear NF-кB concentrations for all these mutants (A20 gene knocked-out, IкBα and IкBβ genes double knocked-out, IкBα and IкBϵ genes double knocked-out) exhibit similar single-peaked patterns. In summary, there are two distinctive groups exhibiting two respective patterns of nuclear NF-кB profile response: the first group, consisting of the wild type and the IкBβ and IкBϵ double knocked-out mutant, is dominated by highly oscillatory responses. The second group, consisting of the A20 knocked-out, the IкBα and IкBβ double knocked-out, and the IкBα and IкBϵ double knocked-out mutants, shows mostly single-peaked (non-oscillatory) responses.

In this subsection, we used the SE method to generate distributions of various dynamical responses from a large ensemble of single-cell simulations, and compared those distributions for the wild type and various mutants. We made two key findings. First, there is significant overlap between the distributions of the wild type and mutants. This indicates that two individual cells, even if they are genetically different, can respond to the same stimulus in a similar manner. A better way to characterize the differences induced by the differing genetic conditions is to model a large ensemble of cells and compare the full distributions of single-cell responses. Second, for this biochemical pathway, we observed that distributions of the first Maximum response were the same for any genetic conditions. Similarly, the distributions of the first translocation time responses were the same for the wild type and two of the genetic mutants. This means that some dynamic features are not good indicators of changes in the NF-кB signaling system for genetic comparative studies. The SE approach can be used to screen out bad indicators among the many possible candidates. In the next subsection, we investigate the distributions of dynamic responses for the NF-кB signaling system under two different dosage conditions.

### Statistical ensemble analysis of dosage-dependent NF-кB dynamical behavior

#### Dosage-dependent dynamical behaviors of individual and ensemble-averaged temporal profiles

#### Dosage-dependent distribution of the dynamic features

#### Dosage-dependent distribution of the dynamic patterns

In this subsection, we analyzed the dynamical response of the cellular replicates under two different stimulant dosage conditions. This yielded distributions of six dynamic features and associated dynamic patterns that are descriptive characterizations of the NF-кB signaling system. Unlike the earlier analysis of differential genetic conditions, the differing stimulus dosages generate non-overlapping distributions and clearly distinctive dynamical behaviors. Some of our predictions, e.g., the distribution of first translocation time in Figure 10(B) and dynamic patterns in Figure 11, are experimentally validated [36].

### Sigmoidally shaped SE average of the dose-response curves

*TR = 0*to

*TR = 0.1*in a step-like manner and then by decreasing it from

*TR = 0.1*to

*TR = 0.*If the forward and backward dose-response curves were significantly different, it could be regarded as a sign of hysteresis. In Figure 12, both forward and backward dose-response curves for each replicate look the same, i.e. no hysteresis effects were seen. Figure 12 also shows both the individual dose-response curves and the SE average have a sigmoidal shape, which indicates a switching behavior between on and off states. For a signal strength greater than the inflection point of a dose-response curve, the stationary level quickly reaches a plateau whose value is three orders of magnitude higher than its low stationary level at a signal smaller than the inflection point. Lastly, the cell-to-cell variability in the stationary nuclear NF-кB level is dramatically larger with a weak signal than with a strong signal, i.e., the variation is two orders of magnitude at

*TR = 10*

^{ -4 }and one order of magnitude at

*TR = 0.1*. The variability is a maximum at the inflection point of the SE dose-response curve, where it is roughly four orders of magnitude. The cell-to-cell variability in the location of the inflection point (along the x-axis) is about one order of magnitude.

## Discussion and conclusion

In this paper, we have used a novel statistical ensemble (SE) method to mimic protein dynamics in a population of cells influenced by extrinsic noise. For our model of the NF-кB signaling system, we showed that the SE averages match population-averaged experimental data. The added value of the SE method is that it can also produce entire distributions of response, which can potentially be compared to experimental observations at the single-cell level. The main predictions enabled by the SE method were as follows: (a) nuclear NF-кB concentration profiles for single cells are expected to fall into one of several distinct heterogeneous dynamic patterns, (b) larger dosages should induce more oscillatory dynamic patterns of nuclear NF-кB response, while smaller dosages should primarily induce single-peaked patterns, (c) larger (smaller) dosages should make First translocation times more narrowly-distributed (broadly-distributed) and shift the peak of its distribution to earlier (later) times, and (d) the shape of dose-response curves, both at the single-cell and population level, should be sigmoidal. After making these predictions computationally, our experimental colleagues used single-cell fluorescence imaging to monitor NF-кB nucleo-cytoplasmic translocation dynamics in lipopolysaccharide-insulted murine macrophage cells, and found that nuclear GFP-RelA (a subunit of NF-кB dimers) profiles are very heterogeneous. They also found NF-кB dynamic responses to be much more heterogeneous and less oscillatory when the stimulant dosage was smaller. They also stimulated the murine macrophages with two different dosages (1 nM and 1 μM) of E. Coli lipopolysaccharide and found two distinctly different distributions of NF-кB translocation time [36]. Thus two of our predictions have been verified by our collaborators who are planning to publish the results elsewhere. We hope our computational analyses will elicit more single-cell experimental measurement to verify the predicted dynamic behaviors.

We wish to emphasize that the novelty of our analysis is not due to its methodology, but rather the viewpoint we adopt with regard to computational modeling of cellular response. Most previous modeling efforts have focused on bifurcation analysis of the response of a dynamical system as its input kinetic rate constants are varied. This approach makes a one-to-one correspondence between a form of dynamic response and a single set of parameter values. By contrast, the assumption in the SE approach is that the dynamics of protein response in individual cells is intrinsically heterogeneous. We assume a population of cells (the replicates of the SE) does not occupy a single point, but rather a volume of points in high-dimensional parameter space. We choose a hybercube sub-volume of this space (a midpoint and interval size for each dimension) and sample from it efficiently to assign kinetic parameters to each replicate in the SE. In this regard, our SE approach looks similar to sensitivity analysis by using Latin Hypercube Sampling method. But, our analysis is not for sensitivity analysis of cell signaling systems to perturbation of model parameter values, but for generation of the heterogeneous responses in single cells. As explained below, we choose the bounds of this sub-volume by fitting the resulting SE averages to experimentally observed population-level averages. Once this is done and the averages match, our assumption is that we can understand the heterogeneous behavior of the biochemical network at the single-cell level by analyzing the wealth of distribution data provided by the SE computations across its set of replicates.

The sigmoidal shape of the dose-response curve reveals two important properties of NF-кB signaling: its switching behavior and its monostability (i.e., no hysteresis). The inflection points of individual sigmoidal curves can be viewed as activation thresholds for the NF-кB signaling pathway. As shown in Figure 12, the NF-кB response is quite small for signal strength below the threshold, while the response increases dramatically (log scale on y-axis) for signal strengths just above the threshold. Knowing that some NF-кB target genes are inflammatory cytokines and that over-expressed inflammatory response is harmful to the host, we can speculate that the NF-кB signaling network employs this sigmoidal dose-response curve to down-regulate excessive inflammatory responses, i.e., to only turn on if the danger level is significantly high, otherwise to shut down. We also note that the amplitude and timing of the first response peak for inflammatory cytokines (such as TNFα) are known to be critical in mediating timely and effective immune response. This is motivation for measuring the dosage-dependent transient dynamic response of NF-κB target genes to investigate the shape of the dose-response. Lastly, TNFα autocrine signaling forms a positive feedback loop in the NF-кB signaling network and can induce bistability, which could modify our results indicating monostability.

Our statistical analysis of protein dynamics depends on how accurately the computationally generated ensemble of the NF-кB signaling system represents a true biological population of individual cells. This question is equivalent to what is the true distribution of extrinsic noise? I.e., what is the distribution from which the kinetic input parameters should be sampled? In this paper, we have chosen a simple answer to this question by assuming the distribution is uniform and bounded. We devised a heuristic fitting algorithm to find the bounding limits of the uniform distribution for each kinetic parameter by minimizing the discrepancy between the SE averages and the population-level experimental data. This heuristic scheme could be converted to a more rigorous optimization problem: to find the distribution of kinetic parameters for a network model which minimizes the difference between the SE average and the population-level experimental measurements, while simultaneously reproducing the range of experimentally-observed heterogeneous protein dynamics in single cells.

Additional improvements could also be made to the procedure for sampling from the parameter space. For example, the sampling could become more biologically relevant, by accounting for changes in the distribution of extrinsic noise over time as cells traverse their cell cycle. We have also assumed no correlation between pairs of kinetic parameters. In fact some parameters may be co-dependent because cellular energy resources are limited: e.g., as one kinetic process is accelerated, others may be inhibited to balance cellular energy consumption. All of these computational tasks would be made easier with additional single-cell experimental data from which the true distribution of extrinsic noise could be inferred.

Finally, we note that our analysis in this paper was simplified by categorizing the nuclear NF-кB response profiles into four dynamic patterns. This simplified various statistical analyses and made it easier to characterize changes in the distribution when genes were knocked-out. Our choice was based on mathematical characterization of the dynamic protein profiles. However, it is possible this neglects other biologically important details of the nuclear NF-кB response, e.g. classification by time periodicity or by steady-state level. Since the choice of categories can affect subsequent analysis, this is an important factor to consider when using the SE methodology.

## Methods

### Six dynamic features of nuclear NF-кB profiles

We define six dynamic features to represent the distinguishing characteristics of temporal profiles of nuclear NF-кB concentration. The first translocation time is the time when the first peak occurs; the first period measures the time between the first two peaks; the first and second maxima are the amplitudes of the first and second peaks; the first minimum is the amplitude of the “valley” between the first two peaks; steady state refers to the asymptotic amplitude at sufficiently long time. Using the first maximum as a reference level, we use scaled ratios, i.e., the first minimum, the second maximum, and the steady state are normalized by the first maximum. The distributions of these dynamical features are presented in Figures 8 and 10.

### Generation of the SE of NF-кB signaling network

Each kinetic rate constant listed in Table 1 is randomly sampled from an interval (*x*_{
0
}*(1-χ), x*_{
0
}*(1 + χ)*) where *χ*_{0} is the reference value and *χ* is a heterogeneity factor. To sample efficiently in the high dimensional space of dozens of parameters, we use the Latin Hypercube Sampling methodology discussed below. For this paper, we used *χ* = 0.3. To generate a statistical ensemble (SE) of *N* replicates, we simply generate *N* sets of randomly sampled kinetic parameters.

### Algorithm to fit the SE average to population-level experimental data

The goal of our fitting algorithm is to determine kinetic parameters that provide the best match for features of the SE average to the experimental time-series data. We do not attempt to fit all of the eighty kinetic parameters. This can result in “over-fitting”, with too many parameters fit to too little data. Also, by sensitivity analysis, others and we have found there are only a handful of kinetic parameters in the NF-кB signaling network whose variation significantly affects the temporal profile of the nuclear NF-кB concentration [23, 35, 40]. Based on our previous studies [35, 40], we choose the two kinetic parameters most highly correlated with each dynamic feature and varied that set of parameters in the fitting procedure. The heuristic steps are as follows. (1) Use an educated guess for initial kinetic parameters and set the heterogeneity factor to *χ* = 039. (2) Generate the SE and resulting protein profiles and calculate the deviation of the six dynamic features of the SE average from the target (experimental) dynamic features. (3) Identify the dynamic feature with largest deviation and modify the two kinetic parameters associated with it. (4) Repeat steps 1-3 until the dynamic features are close to the target values. (5) When a good fit is not achievable decrease *χ* in a step-like manner. All of the data in Figures 3, 5, 4, 7 were obtained through this process.

### Numerical simulation of the NF-кB signaling network

A coupled system of ordinary differential equations (ODEs) is derived from the NF-кB signaling network in Figure 2. Using a 4^{th} order Runge-Kutta scheme, we numerically integrate the ODEs, using initial conditions (i.e., the cytoplasmic NF-кB concentration being equal to the total NF-кB concentration and zero concentrations of all other biochemical species) and kinetic parameters shown in Table 1 and sampled as described below. Before stimulation, the system runs for 33 hours until its constituents reach equilibrium values. Then, we simulate persistent stimulation by turning on the reaction, IKKn → IKKa with a rate TR*K1 and a non-zero constant value of TR. The ChemCell software package is used to carry out part of numerical simulation [41].

### Latin hypercube sampling (LHS)

LHS is a constrained Monte Carlo sampling scheme. Monte Carlo sampling is a commonly-used approach for assessing the uncertainty of a computational model. By sampling repeatedly from the assumed joint probability function of the input variables, and evaluating the response for each sample, the distribution of responses of the model can be estimated. This approach yields reasonable estimates for the distribution of responses, but a large number of samples is needed if there are many input variables, i.e. a high-dimensional input space. A large sample size can be computationally expensive, which motivates an alternative approach, namely LHS. LHS yields more precise estimates of the response distribution with a smaller number of samples from high-dimensional input spaces [42]. Suppose that the model has K kinetic rate variables and we want N samples, where a sample is a set of K values, one per variable. LHS first selects N different values for each of the K variables, by dividing the range of each variable into N non-overlapping intervals on the basis of equal probability. One value from each interval is selected randomly, in accord with the assumed probability density within the interval. The N values for the first kinetic rate variable are then paired in a random manner (equally likely combinations) with the N values of the second variable. These N pairs are combined in a random manner with the N values of the third variable to form N triplets, and so on, until N K-tuples are formed. Each K-tuple becomes a set of kinetic rate parameters for one replicate within the statistical ensemble of N replicates.

## Declarations

### Acknowledgments

This work was supported by the Laboratory Directed Research and Development program at Sandia National Laboratories, a multiprogram laboratory operated by Sandia Corporation, a Lockheed Martin Company, for the United States Department of Energy, under Contract No. DEAC04-94AL85000. This research was supported in part by the National Science Foundation under Grant No. NSF PHY11-25915.

## Authors’ Affiliations

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## Copyright

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.