A genome-scale metabolic flux model of Escherichia coli K–12 derived from the EcoCyc database
BMC Systems Biology volume 8, Article number: 79 (2014)
Constraint-based models of Escherichia coli metabolic flux have played a key role in computational studies of cellular metabolism at the genome scale. We sought to develop a next-generation constraint-based E. coli model that achieved improved phenotypic prediction accuracy while being frequently updated and easy to use. We also sought to compare model predictions with experimental data to highlight open questions in E. coli biology.
We present EcoCyc–18.0–GEM, a genome-scale model of the E. coli K–12 MG1655 metabolic network. The model is automatically generated from the current state of EcoCyc using the MetaFlux software, enabling the release of multiple model updates per year. EcoCyc–18.0–GEM encompasses 1445 genes, 2286 unique metabolic reactions, and 1453 unique metabolites. We demonstrate a three-part validation of the model that breaks new ground in breadth and accuracy: (i) Comparison of simulated growth in aerobic and anaerobic glucose culture with experimental results from chemostat culture and simulation results from the E. coli modeling literature. (ii) Essentiality prediction for the 1445 genes represented in the model, in which EcoCyc–18.0–GEM achieves an improved accuracy of 95.2% in predicting the growth phenotype of experimental gene knockouts. (iii) Nutrient utilization predictions under 431 different media conditions, for which the model achieves an overall accuracy of 80.7%. The model’s derivation from EcoCyc enables query and visualization via the EcoCyc website, facilitating model reuse and validation by inspection. We present an extensive investigation of disagreements between EcoCyc–18.0–GEM predictions and experimental data to highlight areas of interest to E. coli modelers and experimentalists, including 70 incorrect predictions of gene essentiality on glucose, 80 incorrect predictions of gene essentiality on glycerol, and 83 incorrect predictions of nutrient utilization.
Significant advantages can be derived from the combination of model organism databases and flux balance modeling represented by MetaFlux. Interpretation of the EcoCyc database as a flux balance model results in a highly accurate metabolic model and provides a rigorous consistency check for information stored in the database.
Constraint-based modeling techniques such as flux balance analysis (FBA) have become central to systems biology [1, 2], enabling a wealth of informative simulations of cellular metabolism. Many constraint-based modeling techniques have been first demonstrated for the Escherichia coli K–12 MG1655 metabolic network. A series of E. coli constraint-based models have been published by the group of B. Palsson [3–6], extending work on stoichiometric constraint-based modeling of E. coli dating back more than twenty years [7–10]. These models constitute a gold standard for E. coli modeling, and have seen a range of applications [11–13] including metabolic engineering, model-driven discovery, cellular-phenotype prediction, analysis of metabolic network properties, studies of evolutionary processes, and modeling of interspecies interactions.
Motivated by the widespread use of E. coli metabolic models, we aimed to illustrate the benefits of integrating metabolic modeling into model organism databases by developing an E. coli model derived directly from the EcoCyc bioinformatics database . First, we aimed to use the extensive biochemical literature referenced in EcoCyc to develop a model with improved accuracy for phenotypic prediction, specifically for predicting the phenotypes of gene knock-outs, and for predicting growth or lack thereof under different nutrient conditions.
Second, we sought to make the model easy to understand and operate. Our goal was a high level of model accessibility and readability through (a) tight web-based integration of the model with extensive model query and visualization tools, and (b) a model representation that captures extensive information that enriches the model and aids its understanding, such as metabolic pathways, chemical structures, and genetic regulatory information. Metabolic models are not just mathematical entities that output predictions; they are also artifacts that scientists interact with in multiple ways. If a model can be quickly and easily understood, scientists are more likely to trust its predictions, and the model is easier to reuse, to modify and extend, to learn from, and to validate through inspection. These aspects of a metabolic model depend strongly on how the model is represented, on the software tools available to interactively inspect the model, and on how tightly integrated the model is with those software tools.
Third, we sought to produce a model that is frequently updated to integrate new knowledge of E. coli metabolism.
Fourth, we sought to use the EcoCyc-derived E. coli metabolic model to identify errors in EcoCyc, and open problems in E. coli biology, by performing in-depth investigations of the disagreements between the phenotypic predictions of the model and experimental results.
We present EcoCyc–18.0–GEM, a constraint-based genome-scale metabolic model for E. coli K–12 MG1655 that is directly derived from the EcoCyc model organism database ( http://EcoCyc.org) built on the genome sequence of E. coli K–12 MG1655. The model is implemented using the MetaFlux  component of the Pathway Tools software .
Results and discussion
The EcoCyc–18.0–GEM generated from EcoCyc 18.0 encompasses 1445 genes, 2286 unique cytosolic and periplasmic reactions, and 1453 unique metabolites. Table 1 compares the statistics of EcoCyc–18.0–GEM with previous E. coli metabolic models. EcoCyc–18.0–GEM is an advance over previous stoichiometric models of E. coli metabolism in four respects: in its size; in its accuracy; in its form, readability and accessibility; and in its update frequency. Here we summarize these results; these points will be expanded in subsequent subsections.
The MetaFlux component of Pathway Tools translates Pathway/Genome Database (PGDB) reactions and compounds into constraint-based metabolic models. Our methodology of fusing systems-biology models and bioinformatics databases has several advantages because of strong synergies between these approaches. Databases and models both require extensive literature-based curation and refinement. It is more efficient to perform that curation once in a manner that benefits a database and a model, than to duplicate curation efforts for a database project and a modeling project. Furthermore, the modeling process identifies errors, omissions, and inconsistencies in the description of a metabolic model, and therefore drives correction and further curation of the database if the two efforts are coupled. We made more than 80 EcoCyc updates as a result of comparing model predictions with experimental data and literature for this work. In addition, bioinformatics database curation methods such as the use of evidence codes and citations to provide data provenance, and the incorporation of mini-review summaries that describe enzymes and pathways, benefit systems-biology models, which typically lack data provenance and explanations.
Advances in model size
Compared with iJO1366, EcoCyc–18.0–GEM represents a 6% increase in the number of genes, a 23% increase in the number of unique cytosolic and periplasmic reactions, and a 28% increase in the number of unique metabolites. The size of EcoCyc–18.0–GEM is currently exceeded only by the more mathematically complex ME-model of O’Brien et al., which includes simulation of gene expression, transcriptional regulation, and protein synthesis.
Advances in model accuracy
We conducted a threephase validation of EcoCyc–18.0–GEM to assess its accuracy (see Table 1). In phase I we compared simulated EcoCyc–18.0–GEM rates of nutrient uptake and product secretion in aerobic and anaerobic glucose culture with experimental rates derived from chemostat culture; the performance of EcoCyc–18.0–GEM was equivalent to previous models. In phase II we compared essentiality prediction for all 1445 genes involved in the model with experimental gene essentiality datasets; its error rate in predicting gene-knockout phenotypes decreased by 46% over the best previous model. In phase III we compared nutrient utilization predictions of EcoCyc–18.0–GEM with 431 experimental nutrient utilization tests; its accuracy in predicting growth and respiration under different nutrient conditions increased by 4.8% over previous models as the number of nutrient conditions expanded 2.5-fold. We investigated conflicts between experimental results and predictions of EcoCyc–18.0–GEM in detail, and provide an extensive discussion of these conflicts within the context of EcoCyc and the literature.
Subjects of particular interest include alternative catalytic routes capable of replacing genes thought to be essential; compounds with unclear routes of catabolism which are capable of supporting growth and/or cellular respiration; regulatory and environmental perturbations of the stoichiometric network model; and investigations of what, exactly, constitutes gene essentiality.
Advances in model form, readability, and accessibility
Another benefit of coupling systems-biology models with databases, and a corresponding advance of our model, is that generating a constraint-based model from a database that has associated web-based visualization tools leads to a literate model (by analogy to Knuth’s notion of literate programming ). A literate model is easy to read, and is highly accessible to and understandable by scientists.
Advances in model update frequency
Because the MetaFlux component of Pathway Tools generates constraint-based models directly from the EcoCyc PGDBs, as the database is refined through new curation, those refinements are automatically incorporated into newly generated versions of the model. We release new versions of the EcoCyc-based model three times per year; previous models were updated every four years [4–6]. Although there are reasons to limit the frequency of releases in order to tie them to a well-defined version of the database and throughly test the accuracy of new versions, we believe that more frequent model updates are useful for an organism as important as E. coli.
Validation of biomass metabolites, nutrients, and secretions
Refinement of EcoCyc–18.0–GEM began with the validation of the biomass, nutrient, and secretion metabolite sets, which are detailed at length in Additional file 1 and Additional file 2: Table S1. The biomass metabolite set establishes requirements for growth and determines the growth rate of the simulation. The biomass metabolite set for EcoCyc–18.0–GEM was based on the iJO1366 wild-type and core biomass reaction sets published by Orth et al., with several revisions stemming from differences in content and functionality between EcoCyc and the iJO1366 model. Gene essentiality in constraint-based models is principally determined by the biomass demands of the cell. Inclusion of a metabolite in the biomass metabolite set forces the genes required for manufacture of that metabolite to become essential in the simulation.
A wild-type biomass metabolite set, which is derived from measurement of the biomolecular composition of healthy, growing cells, is not representative of the minimal set of biomass metabolites required for cell survival. Because biomass metabolites not truly required for cell survival will generate false simulation predictions of essentiality in their biosynthetic pathways, the concept of a core biomass metabolite set was developed by Feist et al. The core biomass metabolite set is a biomass metabolite set that is defined with the aim of maintaining quantitative accuracy with regards to cell performance while predicting the observed experimental essentiality data as accurately as possible. Because much of this work focuses on testing the minimum requirements for cell growth, we frequently employed the core biomass metabolite set in our simulations. We use the term “expanded biomass set” to refer to our version of the wild-type biomass metabolite set described in Orth et al., because we do not wish to imply that the simulated cells always represented the wild-type state.
The biomass metabolite sets described here underwent several revisions reflecting differences in scope between EcoCyc–18.0–GEM and iJO1366. Whereas iJO1366 is a purpose-built model developed using the COBRA Toolbox with input from KEGG, EcoCyc, and other databases, EcoCyc is a database with its own schema whose entries are programmatically transformed into an FBA model. The specific metabolites present in iJO1366 therefore cannot always be matched with the specific metabolites generated from EcoCyc by MetaFlux on a one-to-one basis. Several biomass metabolites represented as distinct within iJO1366, such as phosphatidylethanolamines with different chain lengths and saturations, are summed under the heading of a single representative metabolite in the EcoCyc–18.0–GEM biomass set. Additionally, not all processes covered in EcoCyc–18.0–GEM are covered in iJO1366, and the reverse is also true. As a result, the biomass metabolite sets differ slightly. Additional file 2: Table S2 contains a complete side-by-side comparison of the EcoCyc–18.0–GEM and iJO1366 biomass metabolite sets, and lists the differences between them.
We constructed standard nutrient sets based on culture conditions reflecting experiments in glucose or glycerol minimal media and on the model’s capability to use substrates. Those substrates include glucose or glycerol as appropriate, O 2, , phosphate, sulfate, ferrous iron, water, CO 2, minerals appropriate to the biomass objective function, and MOPS buffer (usable as a sulfur source) where appropriate. Because of passive diffusion at the high concentration of ammonium used in experimental culture, is supplied directly in the cytosol (avoiding false negative essentiality predictions for the high-affinity nitrogen transporter amtB), whereas all other nutrients are supplied in the periplasmic space.
Finally, we developed a large set of secreted compounds that could be supplied across all growth conditions explored with our model. It contains both plausible products of E. coli metabolism and dead-end metabolites  within the model. The presence or absence of metabolites in this set should not be construed to indicate their presence or absence in E. coli culture media.
We verified the metabolic reachability of each component within the EcoCyc biomass metabolite set by supplying nutrients representing an aerobic glucose minimal medium and setting the production of each individual metabolite in turn as the optimization goal of MetaFlux, and repaired gaps by means of literature-based manual curation of EcoCyc and expansion of the relevant metabolite sets.
ATP maximization validation
We next confirmed that simulations of aerobic growth on glucose run with maximization of ATP production as their objective made appropriate use of the glycolytic and TCA cycle pathways and agreed with previous work on E. coli FBA.
The maximization of ATP production under aerobic conditions was studied by setting the ATP consumption reaction ATP + H 2O → ADP + Pi + H + as the objective function to be maximized by MetaFlux. The fluxes resulting from the maximization of ATP production on glucose under aerobic conditions were compared with fluxes from COBRA Toolbox  simulations of iJO1366 under the same conditions and were found to be largely identical (Table 2). Differences arose from variances in proton translocation stoichiometries between the EcoCyc–18.0–GEM version of the NADH:ubiquinone oxidoreductase I (NADH-DEHYDROG-A-RXN) (4 H + translocated per 2 e -, as proposed by Treberg et al.) and the iJO1366 version of the NADH:ubiquinone oxidoreductase (NADH16pp) (3 H + translocated per 2 e -, as proposed by Wikstrom and Hummer ). The exact number of protons translocated by the NADH:ubiquinone oxidoreductase is an issue of open discussion in the scientific literature, and this uncertainty is described in the EcoCyc summary for the enzyme. If a consensus develops behind the 3 H + per 2 e - view of translocation stoichiometry, future versions of EcoCyc will be changed to reflect this fact.
Further numerical differences are due to a technical consideration: EcoCyc cytochrome bo oxidase reaction stoichiometry is written in terms of whole molecules of oxygen, while iJO1366 CYTBO3_4pp is written in terms of half-molecules (1 O 2 consumed vs. 0.5 O 2).
Comparison with iJO1366 simulation and chemostat data
After completing our basic validation of biomass production and energy generation, we maximized the rate of EcoCyc–18.0–GEM biomass metabolite set production under several minimal media conditions and ensured that we obtained results comparable to the iJO1366 results for the same conditions obtained using the COBRA Toolbox. Divergences were addressed by literature-based manual curation of EcoCyc and modification of MetaFlux reaction sets. We further compared the extracellular flux distributions resulting from these simulations with the experimental data obtained in carbon-limited chemostat environments under both aerobic and anaerobic conditions.
Tables 3 and 4 compare extracellular metabolite flux results derived from EcoCyc–18.0–GEM simulation, iJO1366 simulation, and experimental data [23, 24] for the canonical cases of aerobic and anaerobic growth on glucose-limited chemostat culture. In all simulations, the experimental rate of glucose supply was the only fixed constraint; all other nutrients and secretions were left unconstrained.
The behavior of MetaFlux/EcoCyc–18.0–GEM simulations was very similar in most regards to the behavior of COBRA/iJO1366 simulations. Respiration and fermentation rates scaled with nutrient uptake at comparable rates. The generally higher rates of O 2 uptake observed experimentally lend support to a lower practical efficiency of proton translation stoichiometry in vivo, perhaps augmented by respiratory inefficiencies such as futile cycling and generation of reactive oxygen species. Both models secrete the expected 1:2:1 mix of acetate, formate, and ethanol during anaerobic growth on glucose that Varma et al. originally identified as stoichiometrically optimal. During the transition between purely anaerobic and aerobic domains, the competing demands of energy metabolism and redox elimination cause a characteristic pattern of mixed acid fermentation described by Varma et al., in which ethanol, then formate, and finally acetate production are eliminated as the cell’s oxygen supply becomes completely sufficient to support aerobic respiration. Figures 1 and 2 use the Cellular Overview and Omics Popup visualization functionalities of Pathway Tools to illustrate this behavior in EcoCyc–18.0–GEM during a transition from anaerobicity to aerobicity.
Comparisons between FBA-predicted extracellular fluxes and experimental fluxes show that EcoCyc–18.0–GEM and iJO1366 FBA predictions agree more closely with each other than with experimental flux results, although the correspondence between simulation and experiment was quite close for the experimental fluxes under consideration. This result was expected given the adaptation of the iJO1366 biomass function for use in EcoCyc–18.0–GEM, the use of iJO1366 and preceding reconstructions as benchmarks in the development of EcoCyc–18.0–GEM, and the use of EcoCyc as a reference in the construction of iJO1366 and its predecessors. The experimental measurements generally demonstrate higher fluxes of the respiratory gases O 2 and CO 2 than the simulated fluxes, suggesting a degree of respiratory inefficiency not properly modeled by FBA. Similarly, small quantities of succinate and lactate were produced by experimental fermentation, indicating a degree of divergence from metabolic optimality in vivo. Broader cellular constraints such as regulation, protein crowding, pathway enzyme synthesis requirements, and pathway-throughput limits underlie these differences [17, 26, 27]. Successive generations of evolution under constant growth conditions might bring the experimental result closer to theory, as described in Ibarra et al..
Gene essentiality analysis
One of the most exciting aspects of genome-scale flux modeling is the ability to rapidly test computational gene knockouts (KOs) for their effects on metabolic function. Gene KO simulation is useful both for prediction and for validation: in silico FBA screens of gene KOs have been applied in a variety of metabolic engineering efforts [29–31], and E. coli KO library collections with well-characterized growth behavior provide an important tool for flux model validation.
FBA gene KO essentiality prediction depends on two types of database associations between genes and chemical reactions: genes whose products catalyze reactions, and genes whose products are reaction substrates (e.g., acyl-carrier protein). Simulation gene KOs are carried out by identifying all reactions involving the gene, and then identifying all other genes capable of catalyzing the reactions or supplying the substrates thus identified. Reactions for which no isozymes or alternative substrate supplies are found are removed from the FBA stoichiometric network. An FBA solution is then calculated for the new model. If the simulated gene KO has caused the deletion of one or more reactions required for the synthesis of a biomass metabolite, generation of the full biomass metabolite set will be blocked and the FBA simulation returns a no-growth result. Such a result represents a prediction of gene essentiality. If the complete biomass metabolite set can still be produced in spite of the simulated gene knockout, the FBA simulation returns a growth result, indicating a prediction of gene non-essentiality.
The experimental essentiality data used in our tests consisted of two major datasets. The first, used to study gene essentiality on rich and glucose minimal media, was the deletion study of Baba et al. as updated by Yamamoto et al., which tested the Keio collection library of 4288 E. coli gene deletion strains for growth on LB rich media and MOPS minimal media with 0.4% glucose. We conducted our glycerol minimal media tests using the gene knockout essentiality data of Joyce et al., an expansion of the study of the Keio collection essentiality to include growth on M9 minimal medium with 1% glycerol.
Several E. coli gene deletions strongly affect growth on various types of minimal media, but are nonessential to growth on rich media. Because the FBA simulation result is treated as a binary test (growth or no-growth), gene deletions that strongly affect growth on minimal media without producing a completely lethal phenotype must be defined either as experimentally essential or as experimentally nonessential.
Two representative perspectives on this definition are the narrow essentiality criteria of no observable growth in minimal media and the broad essentiality criteria used by Orth et al. The narrow glucose essentiality criteria treat as essential those Baba et al. and Yamamoto et al. gene deletion mutants with OD600 ≤ 0.005 after 24 and 48 hr growth on glucose minimal media. This requires no perceptible growth over a long period. The broad essentiality criteria was originally defined in relative terms by Joyce et al., as the slowest-growing ninth of all Keio collection deletion mutants.
In absolute terms, that approach treats as essential those deletion mutants measured by Baba et al. to have OD ≤ 0.091 after 24 hr growth on glucose minimal media, which indicates impaired growth over a shorter period. The practical difference between these two perspectives is illustrated in Figure 3, which displays the distribution of OD600 data for all rich media-viable Keio collection mutants after 24 hr of growth on MOPS media containing 0.4% glucose, as originally published in Supplementary Table three of Baba et al. As the figure illustrates, the broad essentiality criteria include a population of cells with severe growth defects that is not contained in the narrow essentiality data. The comparison between narrow and broad essentiality criteria can be expanded to glycerol minimal media by comparing narrow glycerol essentiality criteria of no observed growth on rich media with the glycerol essentiality criteria of Orth et al., again derived from the criteria of Joyce et al. involving successive division into thirds.
In order to examine criteria for experimental gene essentiality more deeply and to illustrate the effect of defining a core biomass metabolite set, we conducted essentiality testing using both the expanded and core biomass metabolite sets proposed by Orth et al. Differences in essentiality predictions between the two data sets illustrated the differences between standard cell composition under nominal conditions and the minimal composition required for cell growth.
We simulated single gene KOs on glucose and glycerol minimal media for the 1445 genes in EcoCyc–18.0–GEM to test whether the resulting EcoCyc–18.0–GEM gene deletion mutants were capable of generating core and expanded biomass metabolite sets from sets of nutrients based on the experimental culture media of Baba et al. and Joyce et al. Gene KO simulations capable of generating any growth at all were scored as nonessential, whereas gene KOs blocking generation of the biomass metabolite set were scored as essential.
We compared the results of this simulated essentiality screen with experimental gene essentiality results based on both narrow and broad gene essentiality criteria. Incorrect essentiality predictions were addressed by literature-based manual curation of EcoCyc and modification of MetaFlux metabolite sets. Final essentiality prediction results after curation are summarized in Tables 5 for glucose and 6 for glycerol. The overall accuracy of prediction for growth on glucose with the core biomass metabolite set and broad essentiality criteria was 1375/1445 (95.2% accuracy, 99.0% sensitivity, 77.5% specificity). For prediction of growth on glycerol under the same simulation conditions, the overall accuracy of prediction was 1365/1445 (94.5% accuracy, 98.1% sensitivity, 77.5% specificity). Sensitivity here refers to the percentage of gene deletions resulting in growth that are correctly identified by simulation, while specificity refers to the percentage of gene deletions resulting in no growth that are correctly identified by simulation.
Tables 5 and 6 illustrate that the gene essentiality predictions in EcoCyc–18.0–GEM differed in a number of cases from the gene essentiality conclusions generated by high-throughput gene KO screening. Because these are situations of considerable interest to the development of EcoCyc as a reference, we examined them in greater detail for the case of growth on glucose, with reference to the E. coli literature. Our examination covered two types of incorrect gene deletion growth predictions. The first type was a false positive growth prediction. These genes, which are experimentally essential under the conditions tested by Baba et al., were predicted to be nonessential by EcoCyc–18.0–GEM. The second type was a false negative growth prediction. These genes, which are not experimentally essential under the conditions tested by Baba et al., were predicted to be essential by EcoCyc–18.0–GEM.
Tables 7, 8, 9, 10, 11 and 12 present five broad categories of incorrect gene deletion predictions from EcoCyc–18.0–GEM. Table 7 cover false predictions involving open questions of E. coli biology, false predictions resulting from interesting facets of experimental or simulation methods, and other situations of special relevance. Table 8 covers false predictions in core glycolytic, pentose phosphate, Entner-Doudoroff, and TCA cycle metabolism. This highly interconnected region of E. coli metabolism contains several isozymes and opportunities for reversibility, and presents a challenge to FBA essentiality predictions in the absence of complete regulatory modeling. Table 9 cover false predictions that are the result of unmodeled regulation of gene expression or enzyme activity. Genes repressed under Baba et al. experimental growth conditions, insufficiently expressed isozymes, and cases of enzyme inhibition all fall into this category. Table 10 covers situations in which the essentiality conclusions of the high-throughput essentiality screen differed significantly from the essentiality conclusions made by the E. coli K–12 literature. Table 11 covers false gene essentiality predictions relating to systems beyond the scope of EcoCyc–18.0–GEM’s biomass objective function. Finally, Table 12 covers false gene essentiality predictions made as a result of MetaFlux and EcoCyc technical problems discovered in the course of this study.
Several of the false gene essentiality predictions described within these tables were discussed in the work of Kim and Copley, who examined the essentiality conclusions of Baba et al. in E. coli core metabolism with reference to the then-current state of EcoCyc. Constraint-based model improvement and gap-filling based on gene essentiality predictions derived from the work of Baba et al. have been examined for the COBRA family of constraint-based models of E. coli metabolism by Reed et al., Kumar et al., Kumar and Maranas , Barua et al., Orth and Palsson [73, 144], and Tervo and Reed . Our revisions of EcoCyc–18.0–GEM included manual application of a subset of GrowMatch  gap-filling methods, specifically resolution of false positive gene essentiality predictions associated with blocked genes and false negative results associated with secretion of metabolites. The essentiality prediction accuracy resulting from our manual curation process is similar to the accuracy resulting from applying the full GrowMatch algorithm to the iAF1260 model.
Additional file 2: Table S3 provides detailed listings of essentiality status and model predictions, including a breakdown of gene essentiality prediction status by criteria used.
Nutrient utilization analysis
Observation of culture growth on various nutrient sources is a foundation of microbiology . EcoCyc 18.0 contains information on E. coli respiration for 428 types of media, including 22 conventional types of minimal growth media and 383 Biolog Phenotype Microarray (PM) wells. The 383 Biolog PM media conditions represent a high-throughput method of evaluating metabolic phenotypes in culture based on a tetrazolium dye assay of cellular respiration. Each well in a Biolog 96-well PM plate contains a standard minimal media composition plus a nutrient source that is varied across the PM plate, with the element supplied by the varying nutrient source dependent on the type of Biolog PM plate in use [147–149].
We evaluated the performance of EcoCyc–18.0–GEM in predicting growth for the following available datasets: (1) aerobic E. coli growth on the 22 common conventional minimal growth media; (2) consensus estimates of respiration based on four different experimentalists’ measurements of aerobic Biolog 96-well plates PM1–4, representing 313 conflict-free growth observations; and (3) an anaerobic Biolog PM1 plate assay surveying carbon source utilization in the absence of oxygen, representing 96 anaerobic growth observations. Biolog PM data stored in EcoCyc measures utilization of nutrients as sources of carbon (PM1–2), nitrogen (PM3), sulfur (PM4), and phosphorus (PM4).
Conventional media compositions and growth results were drawn from the literature. Aerobic Biolog PM nutrient utilization assay results were compiled from four different datasets captured in EcoCyc: (1) from our own experiments; (2) from a dataset obtained from B. Bochner; and from the recent publications of (3) AbuOun et al. and (4) Yoon et al.. Anaerobic Biolog PM nutrient utilization assay results were obtained from B. Bochner. We did not include the data of Baumler et al. in our analysis of Biolog PM results because of variation in culture conditions and a high degree of conflict with other datasets under both aerobic and anaerobic conditions . See the Methods section for additional details.
Growth on a given type of media was tested by constructing simulated MetaFlux nutrient sets corresponding to the contents of the media in question and comparing EcoCyc–18.0–GEM growth predictions with experimental growth results. Due to the absence of enterobactin iron uptake modeling in EcoCyc, Fe 3+ in the medium was replaced with Fe 2+. Anaerobic simulations were prepared identically to those performed for aerobic growth, except for the removal of oxygen from the nutrient set, inclusion of the formate-hydrogen lyase reaction, and the removal of the protoheme and pyridoxal 5’-phosphate synthesis requirement from the biomass.
Literature-based EcoCyc curation and appropriate modifications of MetaFlux metabolite sets were used to address incorrect nutrient utilization predictions. The final results for PM array validation after curation are listed in Table 13. Overall accuracy of growth prediction for aerobic Biolog PM assays was 252/313 (80.5%), with 70 assays not evaluated because of experimental conflicts (see the Methods section). Anaerobic Biolog PM assay predictions had an overall accuracy of 74/96 (77.1%). Aerobic growth tests on conventional minimal media contained in EcoCyc had an overall accuracy of 22/22 (100.0%).
The overall accuracy of nutrient utilization prediction across all aerobic and anaerobic PM and conventional growth media is 348/431 (80.7%). Tables 14, 15, 16 and 17 provide detailed discussions of false negatives and false positives for aerobic PM assays. Tables of results for anaerobic PM assays and conventional growth media are available in Additional file 2: Tables S8 and S9, respectively.
Model readability and accessibility
Scientists naturally need to ask many questions of a metabolic model, such as “What are the chemical structures of all substrates in reaction X, and is X chemically balanced?” “What metabolic pathway(s) is reaction X a member of, and what are the adjacent reactions?” “Which E. coli enzymes are inhibited by ADP?” “What transcriptional regulators affect the expression of the enzymes for reaction X?” Their ability to answer these questions rapidly and accurately is strongly dependent on the model representation, the software tools available for querying and visualizing that representation, the tightness with which those tools are integrated with the model, and the presence of additional enriching information for the model.
Existing E. coli models are represented as spreadsheet files and as SBML files, making it tedious or impossible for non-programmers to answer the preceding questions directly from those files. Although SBML files can be imported into software tools such as the RAVEN Toolbox  and rbionet , in practice that approach is limited because of variations in SBML encodings, the effort required to install and integrate multiple software tools with disparate capabilities, and the limited visualization capabilities of those tools. More fundamentally, previous E. coli models do not capture (nor can SBML capture) additional enriching information that, while not required for the mathematical operation of a model, greatly enhances our ability to validate and understand a model, and to answer the preceding questions. Examples of such enriching information present in EcoCyc–18.0–GEM are metabolite chemical structures, arrangements of reactions within metabolic pathways, and gene regulatory information. Note that introducing ad-hoc definitions of these data (e.g., pathways) in the SBML “Notes” field, or introducing SBML links to external databases, would be considered out of bounds: since pathways are not captured formally in the SBML specification today, there is no guarantee regarding interoperability of software tools with such ad-hoc data.
EcoCyc–18.0–GEM is highly understandable because it can be interactively queried and visualized through the EcoCyc web site and desktop Pathway Tools software, which supports visualization of metabolic pathways and reaction diagrams; metabolite pages that depict metabolite structures and all reactions a metabolite is involved in; depiction of gene/reaction connections and of genome organization via a genome browser; navigation through the E. coli gene regulatory network; constructing structured queries such as: find all reactions of a given metabolite; find all enzymes utilizing a given cofactor; and presentation of text summaries and citations that explain and support aspects of the model. In general, other tools for metabolic model visualization tend to be less comprehensive, and to be less closely coupled to the model; see [183–186] for recent reviews.
The reaction fluxes computed from EcoCyc–18.0–GEM are more understandable than those from previous E. coli models because EcoCyc–18.0–GEM fluxes can be immediately painted onto the EcoCyc Cellular Overview, a zoomable diagram of the complete metabolic map of E. coli that allows immediate visual inspection of flux patterns. Although other software tools exist for visualizing flux patterns on metabolic networks, e.g., the RAVEN Toolbox, they are unlikely to be easily usable with previous E. coli models. For example, RAVEN Toolbox requires that the user manually construct the metabolic network diagram, which could take days or weeks of effort. In contrast, Pathway Tools generates metabolic map diagrams algorithmically from a PGDB.
EcoCyc–18.0–GEM demonstrates the advantages of literate modeling based on comprehensive organism databases. It provides comprehensive genome-scale coverage of the E. coli metabolic network, representing gene function with an unprecedented degree of accuracy.
Integration of EcoCyc–18.0–GEM into the EcoCyc database gives investigators working with the model access to the full Pathway Tools bioinformatics and data visualization suite. This allows construction of complex database queries involving the full range of biochemical entities within E. coli, and visualization of pathways and reactions within the model as they change throughout the course of construction. As part of EcoCyc, EcoCyc–18.0–GEM will receive frequent updates to remain abreast of recent research developments.
The process of EcoCyc–18.0–GEM construction and validation resulted in more than 80 updates to EcoCyc. These included expansion and revision of periplasmic phosphatase activities many updates to sugar transport and phosphotransferase system modeling; correction of incorrect compartment assignments; fixes for L-lactate dehydrogenase action; revisions to glutathione hydrolysis; new transport reactions for compounds identified as nutrient sources during Biolog PM testing; addition of MOPS catabolism via the alkanesulfonate pathway; removal of several incorrect reactions and gene-protein relationships; numerous fixes to reaction reversibility and directionality; several compound class reassignments to correct issues with reaction instantiation; mass rebalancing for several reactions; and revisions to ATP synthase proton stoichiometry. These updates are outlined in Additional file 2: Table S11.
The MetaFlux software has also been improved as a result of the FBA validation of EcoCyc. These improvements include upgrades to compartmentalization handling, gene deletion code, and electron transfer reaction handling. MetaFlux solution and log files have been updated to contain additional statistical information and provide a more detailed explanation of the metabolic network construction process to the user. Numerous updates and revisions to the MetaFlux model of E. coli have been introduced as part of this effort. Biomass metabolite sets have been revised to reflect the work of Orth et al., and additional updates have been made on the basis of the validation process in order to create the most accurate final product possible.
Many questions of interest to E. coli modelers and experimentalists were raised in the course of EcoCyc–18.0–GEM development. By highlighting these questions and presenting them within the context of EcoCyc as a reference database, we address the interests of the general metabolic modeling audience and of E. coli experimentalists interested in using models to explore their results and generate new leads for research. We summarize these questions here.
Experimental measurements of respiratory fluxes in glucose-fed aerobic chemostat culture are higher than those predicted by simulation, and small quantities of succinate and lactate are generated in experimental anaerobic fermentations, suggesting interesting in vivo deviations from theoretical in silico optimality.
A small number of metabolic byproducts must be removed directly from the cytosol in the secretion set because of a lack of known salvage or excretion pathways. The fate of these metabolites is of interest.
Several incorrect essentiality predictions are associated with unclear cellular biomass requirements, pathways with potential alternate routes of catalysis, uncertain determinations of essentiality on glucose minimal media, and ambiguous or missing gene function. Similarly, nutrient utilization predictions have identified a number of compounds that lack clear pathways of entry into metabolism but are capable of supporting respiration and/or growth. Resolution of these uncertainties would improve our understanding of E. coli function in varying environments.
The data used for this project were obtained from EcoCyc version 18.0, and the bioinformatics and flux analysis procedures documented here were performed in either the Web or desktop environment of the Pathway Tools 18.0 software. Pathway Tools can be downloaded, along with documentation and example files, at http://brg.ai.sri.com/ptools/. The simulation tests were constructed by using Lisp scripting and the Pathway Tools Lisp API, documented at http://brg.ai.sri.com/ptools/api/. Further details on the construction of EcoCyc–18.0–GEM can be found within Additional file 1.
EcoCyc–18.0–GEM development employed the MetaFlux component of Pathway Tools, documented in the Pathway Tools User’s Guide and in . All simulations were run in solving mode on a 2.7 GHz i7 MacBook Pro with 16 GB RAM. The “minimize-fluxes: yes” option was used for taxicab norm minimization of fluxes. For additional information, see the Pathway Tools User’s Guide. A MetaFlux.fba file demonstrating simulation of aerobic growth of E. coli BW25113 on glucose is included as Additional file 3.
We note that the choice of stoichiometric representation of equations can affect flux balance solutions when minimization of summed flux is used as part of the objective function; see  for further details. Stoichiometric coefficients within EcoCyc–18.0–GEM are scaled so as to provide minimum whole-integer stoichiometry. Application of different scaling within the flux network may lead to altered flux solutions.
Briefly, MetaFlux creates a stoichiometric metabolic flux network at run time from the metabolites and reactions contained in a Pathway Tools PGDB. During network construction, MetaFlux removes PGDB reactions that are: ambiguously instantiated (see below); unbalanced or having an undetermined balance state; disconnected from the network; marked as physiologically irrelevant; involved in polymerization; involved in polymer segment or protein modification; lacking substrates on one side; containing substrate entities that are described only by strings; possessed of variable stoichiometry; or have more than 10,000 permutations that must be checked during instantiation. MetaFlux then instantiates reactions containing compound classes, replacing the class reactions with mass-balanced reactions containing instances of the relevant compound classes. The resulting set of metabolites and reactions constitutes the metabolic flux network operated on by MetaFlux. Enzymatic reaction and gene-protein relationship data encoded in the PGDB are used to associate the reactions of the network with enzymes and genes as appropriate.
COBRA [20, 188] simulations of the iJO1366 E. coli genome-scale reconstruction were employed in order to validate the EcoCyc FBA simulations and to provide a point of comparison to existing reference models. The iJO1366 simulations were performed using the COBRA Toolbox 5.0.0 within MATLAB R2010a and cobrapy 0.2 within Python 2.7.6. All iJO1366 simulations used taxicab norm minimization of fluxes as described in the documentation for the optimizeCbModel function.
Biomass metabolite sets were constructed using the wild-type and core biomass sets of Orth et al. and modified according to our research findings and experimental data on gene essentiality. The ATP turnover requirement for growth-associated maintenance costs (GAM) was set to 53.95 mmol ATP/gCDW, while the ATP turnover requirement for non-growth-associated maintenance costs (NGAM) was set to 3.15 mmol ATP/gCDW, per Orth et al.
All coefficients of the biomass metabolite set represent millimoles (mmol) of each metabolite required per gram of cell dry weight (gCDW). Coefficients of the nutrient and metabolite sets represent specific uptake and output fluxes, in millimoles of each metabolite supplied per gram of cell dry weight per hour (mmol/gCDW/hr). An overall biomass flux of 1.0 thus represents a specific growth rate μ of 1.0 hr -1 (one new gram of cell dry weight per gram of cell dry weight per hour).
The protocols followed in our PM experiments are as follows: PM plates 1–4 containing 190 sole carbon sources, 95 sole nitrogen sources, 59 sole phosphate sources and 35 sole sulfur sources were used in this analysis. E. coli MG1655 was obtained from the Yale Coli Genetic Stock Center, pre-grown on nutrient agar and used to inoculate the plates following Biolog instructions. The data were collected and analyzed using the OmniLogH PM system, which records the color change every 15 min for each well in the 96 well assay plates. All incubations were performed at 37°C over 48 hr. For the complete details of all PM assay conditions, please refer to the original publications.
The PM nutrient-source assay is an assay of respiration based on the generation of NADH by carbon metabolism and the subsequent reduction of a tetrazolium redox dye by NADH. As such, it does not directly measure either the cell growth simulated by FBA biomass objectives or the uptake of noncarbon sources. However, checkpoint linkage of carbon-source catabolism to nitrogen, phosphorus, and sulfur source catabolism enables the tetrazolium redox dye assay to probe the metabolism of non-carbon sources . Bochner describes the phenomenon of checkpoint linkage as starvation for elemental nutrient source leading to arrest of cellular respiration, due to redox imbalance or alarmone synthesis. We therefore compared PM respiration results directly with FBA growth simulation.
Biolog PM results are stored within the EcoCyc database and are accessible via the Pathway Tools API and EcoCyc website. Individual Biolog PM assay scores for each well were compared across experimental datasets to establish a consensus for comparison with EcoCyc–18.0–GEM simulation. Because simulations of nutrient utilization were scored according to growth or no growth, experimental Biolog PM results indicating ‘normal’ and ‘low’ respiration were combined into a ‘positive’ result. The majority of Biolog PM tests (313/383) displayed a consensus of either respiration or no respiration across all four experimental datasets used. In 70 of 383 cases, no clear consensus could be reached. These divergent cases were omitted from the nutrient-utilization assay validation because no reliable conclusion could be reached regarding the results. See Additional file 2: Table S10 for a list of omitted PM data.
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The authors would like to thank Mario Latendresse and Markus Krummenacker for MetaFlux development and support, and Larry Reitzer for calling our attention to the problem of dapC essentiality. This work was supported by award number U24GM077678 from the National Institute of General Medical Sciences. The content of this article is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of General Medical Sciences or the National Institutes of Health.
DSW, IMK, and PDK receive fees from SRI International commercial licensing of Pathway Tools.
PDK and DSW conceived and designed the experiments. DSW constructed the model and conducted the simulations. DSW analyzed the data. IMK, AM, and DSW curated the database. DSW and PDK wrote the manuscript. AM and ITP performed phenotype microarray experiments. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 3: MetaFlux .fba file demonstrating simulation of aerobic growth of E. coli BW25113 on glucose.(ZIP 3 KB)
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Weaver, D.S., Keseler, I.M., Mackie, A. et al. A genome-scale metabolic flux model of Escherichia coli K–12 derived from the EcoCyc database. BMC Syst Biol 8, 79 (2014). https://doi.org/10.1186/1752-0509-8-79
- Escherichia coli
- Flux balance analysis
- Constraint-based modeling
- Metabolic network reconstruction
- Metabolic modeling
- Genome-scale model
- Gene essentiality
- Systems biology
- Pathway Tools